Fan N, Cutting S, Losick R
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
J Bacteriol. 1992 Feb;174(3):1053-4. doi: 10.1128/jb.174.3.1053-1054.1992.
The sporulation gene spoVK of Bacillus subtilis was cloned by use of the insertional mutation spoVK::Tn917 omega HU8. The spoVK gene was shown to be the site of an incorrectly mapped mutation called spoVJ517. Thus, a separate spoVJ gene as defined by the 517 mutation does not exist and is instead identical with spoVK.
利用插入突变spoVK::Tn917 omega HU8克隆了枯草芽孢杆菌的芽孢形成基因spoVK。结果表明,spoVK基因是一个定位错误的突变位点,该突变被称为spoVJ517。因此,由517突变定义的单独的spoVJ基因并不存在,而是与spoVK相同。