来自竞争性底物进程曲线的动力学参数。在β-内酰胺酶中的应用。
Kinetic parameters from progress curves of competing substrates. Application to beta-lactamases.
作者信息
Waley S G
出版信息
Biochem J. 1983 May 1;211(2):511-3. doi: 10.1042/bj2110511.
Abstract
The use of two substrates, one of them a reporter substrate, is often convenient. The time course of an enzymic reaction with competing substrates is given explicitly in the present paper. Kinetic parameters can be readily obtained. The method is applied to the hydrolysis of benzylpenicillin, with cephalosporin C as the reporter substrate, catalysed by a Pseudomonas beta-lactamase.
摘要
使用两种底物，其中一种为报告底物，通常很方便。本文明确给出了酶与竞争性底物反应的时间进程。动力学参数可以很容易地获得。该方法应用于假单胞菌β-内酰胺酶催化的以头孢菌素C为报告底物的苄青霉素水解反应。
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