Fisher N C, Hinds-Aldrich N, Haddow J E, Hudson G A
Thromb Res. 1983 Jul 1;31(1):145-54. doi: 10.1016/0049-3848(83)90015-4.
We describe a cellulose sandwich fluoroimmunoassay for Factor VIII-related antigen (FVIIIR:Ag). Plasma is incubated with cellulose-antibody for 2 hours, fluorescent-labelled antiserum is added and the samples are allowed to react for an additional 2 hours. The fluorescence associated with the cellulose-antibody is measured after the tubes have been centrifuged, and the deposit washed and then digested with sodium hydroxide. Ninety-seven samples are assayed and the results are compared with radioimmunoassay (correlation coefficient 0.960) or electroimmunoassay (correlation coefficient 0.882). Between-run coefficients of variation are 8.0% to 9.9%. The cellulose-antibody reagent and the fluorescent-labelled antiserum were both stable over a period of 1 year, demonstrating a major advantage of fluoroimmunoassay over radioimmunoassay.
我们描述了一种用于检测因子VIII相关抗原（FVIIIR:Ag）的纤维素夹心荧光免疫测定法。血浆与纤维素-抗体孵育2小时，加入荧光标记抗血清，样品再反应2小时。离心管后，测量与纤维素-抗体相关的荧光，沉淀物洗涤后用氢氧化钠消化。检测了97个样本，并将结果与放射免疫测定法（相关系数0.960）或电免疫测定法（相关系数0.882）进行比较。批间变异系数为8.0%至9.9%。纤维素-抗体试剂和荧光标记抗血清在1年内均保持稳定，这证明了荧光免疫测定法相对于放射免疫测定法的一个主要优势。
