Benková M, Borosková Z, Berezková V K
Vet Med (Praha). 1984 Jan;29(1):39-46.
After experimental invasion of guinea-pigs with the eggs of Ascaris suum (5,000 eggs per animal), the dynamics and distribution of T and B-dependent cells in lymphoid organs (spleen, mesenteric, hepatic and mediastinal nodes) were studied. The detection of both cell populations by means of rosette methods (E, EA, EAC) showed that in all studied organs their first increase took place on the first and second day after invasion. On the fifth to seventh day after invasion the maximum increase in T cells was observed in spleen, mesenteric and hepatic nodes and on the ninth day in mediastinal nodes. After an initial increase, the number of B cells carrying specific receptors for Fc fragment Ig (EA-rosettes) reached its maximum in mesenteric nodes on the fifth day, in spleen on the seventh day and in hepatic and mediastinal nodes on the ninth day after invasion. The maximum number of B cells with receptors for complement (EAC-rosettes) appeared on the seventh day after invasion in spleen and in hepatic and mesenteric nodes, whereas in mediastinal nodes on the ninth day. On the 21st day after invasion the percentage of T and B cells decreased to the initial values. As follows from the results, the dynamics and distribution of T and B-dependent cells, detected in corresponding organs by rosette methods, correlated with the ascaroid type of endogenous migration of Ascaris suum larvae.
在用猪蛔虫卵（每只动物5000个卵）对豚鼠进行实验性感染后，研究了淋巴器官（脾脏、肠系膜、肝和纵隔淋巴结）中T细胞和B细胞依赖性细胞的动态变化及分布。通过玫瑰花结法（E、EA、EAC）检测这两种细胞群体，结果显示在所有研究的器官中，它们在感染后的第一天和第二天首次增加。在感染后的第五至七天，在脾脏、肠系膜和肝淋巴结中观察到T细胞的最大增加，而在纵隔淋巴结中则在第九天出现最大增加。在最初增加之后，携带Fc片段Ig特异性受体的B细胞（EA玫瑰花结）数量在感染后的第五天在肠系膜淋巴结中达到最大值，在第七天在脾脏中达到最大值，在第九天在肝和纵隔淋巴结中达到最大值。具有补体受体的B细胞（EAC玫瑰花结）的最大数量在感染后的第七天出现在脾脏以及肝和肠系膜淋巴结中，而在纵隔淋巴结中则在第九天出现。在感染后的第21天，T细胞和B细胞的百分比降至初始值。从结果可以看出，通过玫瑰花结法在相应器官中检测到的T细胞和B细胞依赖性细胞的动态变化及分布与猪蛔虫幼虫的类蛔虫型内源性迁移相关。
