Rennie R P, Elliott J M, Nardini M A, Thornley J H
J Clin Microbiol. 1984 Nov;20(5):923-6. doi: 10.1128/jcm.20.5.923-926.1984.
Counterimmunoelectrophoresis (CIE) was compared with a cytotoxicity assay and isolation of toxin-producing Clostridium difficile for the diagnosis of antibiotic-associated diarrhea. Standardized criteria for CIE positivity were established as two major precipitin lines with specific C. difficile antitoxin. Using these criteria on 425 fecal specimens, we determined the sensitivity of CIE (80%) to be comparable to that of the cytotoxicity assay (82%). Both methods were highly specific. With carefully controlled techniques, CIE can be used in routine diagnostic laboratories to screen for toxigenic C. difficile.
将对流免疫电泳（CIE）与细胞毒性试验以及产毒素艰难梭菌的分离方法进行比较，以诊断抗生素相关性腹泻。CIE阳性的标准化标准确定为与特异性艰难梭菌抗毒素形成两条主要沉淀线。对425份粪便标本应用这些标准，我们确定CIE的敏感性（80%）与细胞毒性试验的敏感性（82%）相当。两种方法都具有高度特异性。通过严格控制技术，CIE可用于常规诊断实验室筛查产毒艰难梭菌。
