Issekutz A C, Biggar W D
Infect Immun. 1977 Jan;15(1):212-20. doi: 10.1128/iai.15.1.212-220.1977.
The chemotaxis of neutrophils has been shown to be modulated by serum factors, tissue factors, bacterial products, and a host of other substances. In vivo, these factors may act in concert with each other to modify neutrophil movement. We examined the effect of aggregated gamma globulin-activated serum (AS), bacterial factors, and endotoxin either alone or in combination with each other, on human neutrophil chemotaxis. Exposure of neutrophils to AS resulted in deactivation to AS but not to Escherichial coli or Staphylococcus epidermis culture filtrate. Exposure of neutrophils to S. epidermis or E. coli CF or E. coli endotoxin resulted in deactivation to AS or C5a but not to E. coli or S. epidermis culture filtrate. Addition of endotoxin to AS or C5a resulted in inhibition of chemotaxis by untreated neutrophils toward this combination as compared with AS alone. These results suggest that separate mechanisms may be involved when serum or bacterial chemotactic factors initiate human neutrophil chemotaxis. Furthermore, the potent but specific inhibitory effect of endotoxin on chemotaxis toward AS may be of clinical significance.
中性粒细胞的趋化性已被证明会受到血清因子、组织因子、细菌产物及许多其他物质的调节。在体内，这些因子可能相互协同作用以改变中性粒细胞的运动。我们研究了聚集的γ球蛋白激活血清（AS）、细菌因子和内毒素单独或相互组合对人中性粒细胞趋化性的影响。将中性粒细胞暴露于AS会导致对AS失活，但对大肠杆菌或表皮葡萄球菌培养滤液不失活。将中性粒细胞暴露于表皮葡萄球菌或大肠杆菌CF或大肠杆菌内毒素会导致对AS或C5a失活，但对大肠杆菌或表皮葡萄球菌培养滤液不失活。与单独的AS相比，向AS或C5a中添加内毒素会导致未处理的中性粒细胞对这种组合的趋化性受到抑制。这些结果表明，当血清或细菌趋化因子引发人中性粒细胞趋化性时，可能涉及不同的机制。此外，内毒素对朝向AS的趋化性具有强大但特异性的抑制作用可能具有临床意义。
