Artz R P, Jacobson R R, Bullock W E
Clin Exp Immunol. 1980 Aug;41(2):343-52.
The effect of granulomatous infections upon the activity of a T lymphocyte subclass in human peripheral blood that can be induced by concanavalin A (Con A) to function in a suppressor mode was studied. Peripheral blood lymphocytes (PBL) from eleven patients with disseminated mycotic or mycobacterial infections or from controls were preincubated with and without Con A, washed and cultured with allogeneic PBL freshly drawn from healthy donors sensitive to histoplasmin. DNA synthesis was then measured in co-cultures stimulated by Con A, histoplasmin, or by the mixed lymphocyte culture (MLC) reaction alone. As compared with cells preincubated without Con A, the Con A-pretreated cells were significantly less effective in suppressing the responses of normal PBL to histoplasmin (P < 0.01), and in a one-way MLC reaction (P < 0.05). The Con A-induced suppressor activity of PBL from nine patients with localized granulomatous infections did not differ significantly from that exerted by PBL of normal controls in two of the three co-culture systems employed. These studies suggest that either dysfunction or a reduction of the Con A-inducible T-suppressor cell subpopulation in peripheral blood is frequent among patients was disseminated granulomatous infections.
研究了肉芽肿性感染对人外周血中一种T淋巴细胞亚类活性的影响，该亚类可被刀豆球蛋白A（Con A）诱导以抑制模式发挥作用。来自11例播散性真菌或分枝杆菌感染患者或对照组的外周血淋巴细胞（PBL），分别在有或无Con A的情况下进行预孵育，洗涤后与从对组织胞浆菌素敏感的健康供体新鲜采集的同种异体PBL一起培养。然后在由Con A、组织胞浆菌素或单独的混合淋巴细胞培养（MLC）反应刺激的共培养物中测量DNA合成。与未用Con A预孵育的细胞相比，经Con A预处理的细胞在抑制正常PBL对组织胞浆菌素的反应（P < 0.01）以及单向MLC反应（P < 0.05）方面显著低效。在所用的三种共培养系统中的两种中，来自9例局限性肉芽肿性感染患者的PBL的Con A诱导的抑制活性与正常对照组PBL的抑制活性没有显著差异。这些研究表明，播散性肉芽肿性感染患者外周血中Con A诱导的T抑制细胞亚群功能障碍或数量减少很常见。
