Bullock W E
J Immunol. 1976 Oct;117(4):1171-8.
Intravenous infusion of radiolabeled thoracic duct lymphocytes (TDL)2 from normal syngeneic donors to rats experimentally infected with Mycobacterium lepraemurium fails to produce a significant increase of cell output and radioactivity within the thoracic duct lymph. Conversely, ther is a marked increase in cell counts and radioactivity in the thoracic duct lymph of control recipients. Splenectomy of infected rats prior to the infusion significantly increased the ouptut of cells and radioactivity from the TD of these rats although it was not restored to normal. Serial quantitation of radioactivity in lymphoid organs of infected rats after infusion of 51Cr-labeled TDL revealed significantly increased uptake by the spleen as compared with the spleens of controls. Thus, the spleen of infected rats was a major trap for recirculating TDL. TDL were also trapped to a lesser extent by the lymph nodes and liver of infected rats. The circulation of TDL was not disturbed significantly in control rats with massive splenomegaly and red pulp hyperactivity induced by i.p. injection of methyl cellulose. Since murine leprosy preferentially involves the periarteriolar lymphocyte sheaths of the splenic white pulp and paracortical area of lymph nodes, it is suggested that the disturbance of lymphocyte circulation is secondary to pathology within these areas.
将来自正常同基因供体的放射性标记胸导管淋巴细胞（TDL）静脉输注到实验感染鼠麻风杆菌的大鼠体内，并未使胸导管淋巴液中的细胞输出量和放射性显著增加。相反，对照受体的胸导管淋巴液中的细胞计数和放射性有显著增加。在输注前对感染大鼠进行脾切除术，虽未使其恢复正常，但显著增加了这些大鼠胸导管的细胞输出量和放射性。在输注51Cr标记的TDL后，对感染大鼠的淋巴器官中的放射性进行连续定量分析，结果显示与对照大鼠的脾脏相比，感染大鼠脾脏的摄取量显著增加。因此，感染大鼠的脾脏是再循环TDL的主要滞留部位。感染大鼠的淋巴结和肝脏对TDL的滞留程度较小。通过腹腔注射甲基纤维素诱导的有大量脾肿大和红髓增生的对照大鼠中，TDL的循环未受到显著干扰。由于鼠麻风优先累及脾白髓的动脉周围淋巴细胞鞘和淋巴结的副皮质区，因此提示淋巴细胞循环的紊乱是这些区域内病理变化的继发结果。
