Hozumi N, Hawley R G, Murialdo H
Gene. 1981 Mar;13(2):163-172. doi: 10.1016/0378-1119(81)90005-6.
An EcoRI fragment carrying the immunoglobulin C kappa gene and multiple J gene segments from the DNA of the NZB strain of mouse was cloned into lambda Ch 4A DNA. Subsequent characterization of the clone by heteroduplex analysis, restriction-enzyme mapping and DNA sequencing demonstrated that the organization of the J gene segments and the C kappa gene of NZB mouse was similar, if not identical, to that of DNA from the Balb/c strain of mouse. Since the amino acid sequence of the light chain of a plasmacytoma of NZB mouse shows a J region sequence different from that of Balb/c mouse, our results indicate that the new J sequence arose by somatic mutation.
将携带来自新西兰黑（NZB）品系小鼠DNA的免疫球蛋白Cκ基因和多个J基因节段的EcoRI片段克隆到λCh 4A DNA中。随后通过异源双链分析、限制性酶切图谱分析和DNA测序对该克隆进行表征，结果表明，NZB小鼠的J基因节段和Cκ基因的组织与来自Balb/c品系小鼠的DNA相似，即便不完全相同。由于NZB小鼠浆细胞瘤轻链的氨基酸序列显示出与Balb/c小鼠不同的J区序列，我们的结果表明，新的J序列是由体细胞突变产生的。
