Hozumi N, Wu G E, Murialdo H, Roberts L, Vetter D, Fife W L, Whiteley M, Sadowski P
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7019-23. doi: 10.1073/pnas.78.11.7019.
The mouse cell line MOPC 315 is an IgA (lambda II)-producing myeloma. We have studied a derivative of MOPC 315 that secretes normal lambda II chains but no heavy chain. This derivative, MOPC 315-26, was found to contain a rearranged lambda I gene in addition to a rearranged lambda II gene. The rearranged lambda I gene was cloned into bacteriophage lambda DNA and its structure was studied. The lambda I gene was found to have arisen by an aberrant recombination event that resulted in a single base insertion at the site of V-J region joining. In addition, the gene contained numerous point mutations in the vicinity of the junction of the V and J regions. Two point mutations occurred in the donor splice sequence normally used for the removal of the intron between the J and C regions, suggesting that the RNA synthesized from the aberrantly rearranged lambda I gene would be unable to undergo proper RNA splicing.
小鼠细胞系MOPC 315是一种产生IgA（λII）的骨髓瘤。我们研究了MOPC 315的一个衍生物，它分泌正常的λII链但不分泌重链。这个衍生物，MOPC 315-26，除了一个重排的λII基因外，还被发现含有一个重排的λI基因。重排的λI基因被克隆到噬菌体λDNA中并研究了其结构。发现λI基因是由一个异常重组事件产生的，该事件导致在V-J区域连接位点处有一个单碱基插入。此外，该基因在V和J区域交界处附近含有许多点突变。两个点突变发生在通常用于去除J和C区域之间内含子的供体剪接序列中，这表明从异常重排的λI基因合成的RNA将无法进行正确的RNA剪接。
