Study on antiblastomogenic action of epidermal chalones. II. Effect of epidermal chalones on carcinogenesis in the vagina.

The effect of epidermal or liver G1 and G2 chalone-containing extracts (CCE), prepared from rat skin and liver, upon DMBA-induced carcinogenesis in the vagina of BALB/c mice was studied. One per cent solutions of CCE in PBS were administered intravaginally on polyurethane sponges twice a week during the course of intravaginal applications of DMBA or after its completion up to the death of mice. When administered during te course of DMBA treatment, skin CCE prolonged the latency of cervicovaginal tumours in comparison with liver CCE (48.0 +/- 0.37 and 44.8 +/- 0.54 days, respectively; p less than 0.05). When administered after the course of DMBA application, skin CCE prolonged the lifespan of mice bearing cervicovaginal squamous cell carcinomas in comparison with liver CCE (192 +/- 12.3 and 161 +/- 9.0 days, respectively; p less than 0.05). Skin CCE did not possess any general toxic effect on mice and they were bearing CCE applications for a rather a long period without visible side effects.