Reverdy M E, Martra A, Fleurette J
Pathol Biol (Paris). 1984 Jun;32(5 Pt 2):591-5.
We studied the effect of nine soaps and/or antiseptics on the bacterial flora of hands 5 minutes after a surgical scrub. Each agent was used by ten healthy volunteers, free of skin lesions. The following agents were used: chlorhexidine gluconate 4% and 1.5%, povidone iodine 4%, ethanol 70 degrees, isopropanol 70 degrees, a non-antiseptic soap, and another soap followed by either ethanol 70 degrees, isopropanol 70 degrees or a preparation containing H2O2. The surgical scrub procedure varied slightly according to whether or not the agent was soapy and required rinsing. Sampling was carried out using Gaschen's bag method with 400 ml of neutralising solution. Counts were made after 48 hours aerobic incubation at 35 degrees C on tryptic soy agar with 1% Tween 80, and after 8 days anaerobic incubation at 35 degrees C on Brewer's yeast agar with 1% Tween 80. Results were expressed as the log 10 to the number of bacteria per hand. Statistical significance was determined using the Student t test. The greatest reduction in aerobic flora was produced by isopropanol 70 degrees C (1.7 log 10). 1.5 to 0.5 log 10 reductions were produced, in the following decreasing order, by ethanol 70 degrees, povidone iodine, chlorhexidine gluconate 4% and 1.5% and a soap with ethanol 70 degrees. A reduction of less than 0.5 log 10 was produced by a soap with isopropanol 70 degrees, and soaps with H2O2. Similar results were obtained with the facultative anaerobes.
我们研究了九种肥皂和/或防腐剂在外科洗手5分钟后对手部细菌菌群的影响。每种制剂由10名无皮肤损伤的健康志愿者使用。使用的制剂如下：4%和1.5%的葡萄糖酸氯己定、4%的聚维酮碘、70度乙醇、70度异丙醇、一种非抗菌肥皂，以及另一种肥皂后接70度乙醇、70度异丙醇或含过氧化氢的制剂。根据制剂是否含肥皂以及是否需要冲洗，外科洗手程序略有不同。使用加申袋法，加入400毫升中和溶液进行采样。在35℃下于含1%吐温80的胰蛋白胨大豆琼脂上需氧培养48小时后计数，以及在35℃下于含1%吐温80的布鲁尔酵母琼脂上厌氧培养8天后计数。结果以每只手细菌数量的log10表示。使用学生t检验确定统计学显著性。70度异丙醇使需氧菌群减少最多（1.7 log10）。70度乙醇、聚维酮碘、4%和1.5%的葡萄糖酸氯己定以及含70度乙醇的肥皂依次产生1.5至0.5 log10的减少。含70度异丙醇的肥皂以及含过氧化氢的肥皂产生的减少小于0.5 log10。兼性厌氧菌也得到了类似结果。
