Jarvis J D, Wynne C D, Enwright L, Williams J D
J Clin Pathol. 1979 Jul;32(7):732-7. doi: 10.1136/jcp.32.7.732.
Two aspects of handwashing in hospital were considered. A study was carried out to examine the contamination of bar soap and containers, and the use of antiseptic soaps in reducing the resident flora of the skin. Swabs were collected from soap dishes on six wards and from a bacteriology laboratory on four consecutive days. The unmedicated bar soap was replaced by bar soap containing 2.5% povidone-iodine, and further swabs were collected over a period of seven days. Ninety-two isolates from 48 samples were obtained when unmedicated bar soap was used, and nine isolates from 42 samples when povidone-iodine (Betadine) soap was substituted. The number of organisms recovered when povidone-iodine soap was used was much reduced, and Pseudomonas spp were recovered in low numbers on only one occasion. Six laboratory workers took part in a study to compare bar soap with other agents-povidone-iodine soap, povidone-iodine surgical scrub, povidone-iodine alcoholic solution, chlorhexidine surgical scrub, and alcoholic chlorhexidine. Samples were collected after standard washes and after surgical gloves had been worn for 90 minutes. The effect of multiple washes was assessed by samples collected after six washes with the agent under study (three per day) followed by 90 minutes wearing surgical gloves. The average percentage reduction in normal flora obtained indicated that alcoholic chlorhexidine was superior to the other agents.
研究考虑了医院洗手的两个方面。开展了一项研究，以检测条皂及其容器的污染情况，以及使用抗菌皂对减少皮肤常驻菌群的作用。连续四天从六个病房的皂盒以及一个细菌学实验室采集拭子。将未加药的条皂换成含2.5%聚维酮碘的条皂，并在七天内进一步采集拭子。使用未加药条皂时，从48个样本中获得了92株分离菌，而使用聚维酮碘（必妥碘）皂时，从42个样本中获得了9株分离菌。使用聚维酮碘皂时回收的微生物数量大幅减少，仅在一次检测中少量检出假单胞菌属。六名实验室工作人员参与了一项研究，以比较条皂与其他制剂——聚维酮碘皂、聚维酮碘外科 scrub、聚维酮碘酒精溶液、氯己定外科 scrub 和酒精氯己定。在标准洗手后以及佩戴手术手套90分钟后采集样本。通过在使用研究中的制剂进行六次清洗（每天三次）后再佩戴90分钟手术手套后采集的样本，评估多次清洗的效果。所获得的正常菌群平均减少百分比表明，酒精氯己定优于其他制剂。
