Patterson B D, MacRae E A, Ferguson I B
Anal Biochem. 1984 Jun;139(2):487-92. doi: 10.1016/0003-2697(84)90039-3.
Methods for the estimation of hydrogen peroxide in acetone extracts using titanium(IV) are likely to overestimate hydrogen peroxide when applied to plant leaves. Pigments appear to co-precipitate with the titanium complex and cannot be removed by washing with solvents. Fluoride, which specifically removes the color of the titanium-peroxide complex, removes only some of the color from the reactions with plant extracts. This problem has been avoided by extracting tissues with trichloroacetic acid, and measuring peroxide against catalase-treated blanks by its reaction with the complex of titanium(IV) with 4-(2-pyridylazo) resorcinol. Levels of hydrogen peroxide in leaves of a variety of species were found to range from about 0.1 to 0.6 mumol X g-1.
使用钛（IV）估计丙酮提取物中过氧化氢的方法应用于植物叶片时可能会高估过氧化氢含量。色素似乎会与钛络合物共沉淀，且无法通过溶剂洗涤去除。氟化物能特异性去除钛 - 过氧化物络合物的颜色，但只能去除与植物提取物反应产生颜色的一部分。通过用三氯乙酸提取组织，并通过其与钛（IV）和4 - （2 - 吡啶偶氮）间苯二酚的络合物反应，以过氧化氢酶处理的空白对照来测量过氧化物，避免了这个问题。发现多种植物叶片中的过氧化氢含量范围约为0.1至0.6 μmol·g⁻¹。
