Frei A, Zimmermann A, Weigand K
Hepatology. 1984 Sep-Oct;4(5):830-4. doi: 10.1002/hep.1840040505.
To evaluate the diagnostic significance of the collagen Type III (Col 1-3) N-terminal propeptide of procollagen Type III, with respect to activity and degree of liver fibrosis, Col 1-3 serum concentrations were measured in 111 patients with chronic liver diseases and in 60 patients were correlated with liver histology and morphometry. Col 1-3 was measured by a specific radioimmunoassay. Biopsies were read without knowledge of diagnosis. Periportal and intralobular lesions were assessed semiquantitatively by allocating 1 of 4 severity grades to each parameter. All portal areas were measured morphometrically. Compared to 27 normal controls, Col 1-3 concentrations were significantly elevated in patients with untreated chronic active hepatitis, cirrhosis and primary biliary cirrhosis, but not in chronic persistent hepatitis or fatty liver. Morphometrically measured portal tract area significantly correlated with Col 1-3 plasma levels. Among the semiquantitatively measured periportal lesions, the number of fibroblasts exhibited the closest relationship with Col 1-3 levels; there was no relationship between Col 1-3 levels and intralobular lesions. These data suggest that Col 1-3 serum levels reliably reflect the activity and degree of liver fibrosis and are useful along with liver biopsy in follow-up of patients with chronic liver disease.
为评估Ⅲ型前胶原N端前肽（Col 1-3）对肝纤维化活动度和程度的诊断意义，对111例慢性肝病患者和60例对照者检测血清Col 1-3浓度，并与肝组织学和形态计量学结果进行相关性分析。采用特异性放射免疫分析法检测Col 1-3。肝活检病理诊断采用盲法。对汇管区和小叶内病变的各项参数进行半定量评分，分为4个严重程度等级。对所有汇管区进行形态计量学测量。与27例正常对照者相比，未经治疗的慢性活动性肝炎、肝硬化和原发性胆汁性肝硬化患者血清Col 1-3浓度显著升高，而慢性持续性肝炎和脂肪肝患者血清Col 1-3浓度无明显升高。汇管区形态计量学测量面积与Col 1-3血浆水平显著相关。在半定量评估的汇管区病变中，成纤维细胞数量与Col 1-3水平关系最为密切；Col 1-3水平与小叶内病变无相关性。这些数据表明，血清Col 1-3水平可可靠地反映肝纤维化的活动度和程度，在慢性肝病患者的随访中与肝活检一样具有重要价值。
