乙酰胆碱受体的管状晶体
Tubular crystals of acetylcholine receptor.
作者信息
Brisson A, Unwin P N
出版信息
J Cell Biol. 1984 Oct;99(4 Pt 1):1202-11. doi: 10.1083/jcb.99.4.1202.
Abstract
Well-ordered tubular crystals of acetylcholine receptor were obtained from suspensions of Torpedo marmorata receptor-rich vesicles. They are composed of pairs of oppositely oriented molecules arranged on the surface lattice with the symmetry of the plane group p2 (average unit cell dimensions: a = 90 A, b = 162 A, gamma = 117 degrees). The receptor in this lattice has an asymmetric distribution of mass around its perimeter, yet a regular pentagonal shape; thus its five transmembrane subunits appear to have different lengths, but approximately equal cross sections. The tubes grow by lateral aggregation on the vesicle surface of ribbons of the paired molecules. Both ribbons and tubes were sensitive to dispersal by the disulphide reductant, dithiothreitol. This observation and other evidence suggest that the basic pairing interaction in the tubes may be that of the physiological dimer, involving contact between delta-subunits.
摘要
从多纹电鳐富含受体的囊泡悬浮液中获得了排列有序的乙酰胆碱受体管状晶体。它们由成对的反向排列分子组成，这些分子排列在具有平面群p2对称性的表面晶格上（平均晶胞尺寸：a = 90 Å，b = 162 Å，γ = 117°）。该晶格中的受体在其周边具有不对称的质量分布，但呈规则的五边形形状；因此其五个跨膜亚基似乎具有不同的长度，但横截面大致相等。这些管子通过成对分子条带在囊泡表面的侧向聚集而生长。条带和管子都对二硫键还原剂二硫苏糖醇的分散敏感。这一观察结果和其他证据表明，管子中的基本配对相互作用可能是生理二聚体的相互作用，涉及δ亚基之间的接触。
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