同时检测刺激后的人多形核白细胞的天然发光和鲁米诺依赖性发光。

Simultaneous detection of native and luminol-dependent luminescence of stimulated human polymorphonuclear leukocytes.

作者信息

Roschger P, Graninger W, Klima H

出版信息

Biochem Biophys Res Commun. 1984 Sep 28;123(3):1047-53. doi: 10.1016/s0006-291x(84)80239-9.

DOI:10.1016/s0006-291x(84)80239-9

Abstract

A method for investigating the cellular response of polymorphonuclear leukocytes to various stimuli was introduced using simultaneously native (luminol-independent) and luminol dependent luminescence as an indicator for myeloperoxidase (MPO)-H2O2-halide and O2- mediated reactions. In experimental systems containing low concentrations of luminol the total light emission was separated into contributions of native and luminol-dependent luminescence by making use of the different spectral behaviour of the two kinds of luminescence. Consequently the MPO-H2O2-halide system could be distinguished from the O2- dependent system by interpreting the recorded temporal traces of the emitted light.

摘要

介绍了一种利用天然（不依赖鲁米诺）和依赖鲁米诺的发光同时作为髓过氧化物酶（MPO）-H2O2-卤化物和O2-介导反应指标来研究多形核白细胞对各种刺激的细胞反应的方法。在含有低浓度鲁米诺的实验系统中，利用两种发光不同的光谱行为将总发光分离为天然发光和依赖鲁米诺发光的贡献。因此，通过解释记录的发射光的时间轨迹，可以将MPO-H2O2-卤化物系统与O2-依赖系统区分开来。

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