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New biphasic culture system for isolation of mycobacteria from blood of patients with acquired immune deficiency syndrome.用于从获得性免疫缺陷综合征患者血液中分离分枝杆菌的新型双相培养系统。
J Clin Microbiol. 1984 Sep;20(3):572-4. doi: 10.1128/jcm.20.3.572-574.1984.
2
Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia.使用裂解离心法(隔离器)和放射性测量法(BACTEC)血液培养系统检测分枝杆菌血症。
J Clin Microbiol. 1985 Oct;22(4):543-6. doi: 10.1128/jcm.22.4.543-546.1985.
3
Mycobacterium avium: a pathogen of patients with acquired immunodeficiency syndrome.鸟分枝杆菌:获得性免疫缺陷综合征患者的一种病原体。
Diagn Microbiol Infect Dis. 1984 Jun;2(3):213-8. doi: 10.1016/0732-8893(84)90033-6.
4
Comparison of methods for isolating Mycobacterium avium-intracellulare from blood of patients with AIDS.从艾滋病患者血液中分离鸟分枝杆菌-胞内分枝杆菌方法的比较。
J Clin Pathol. 1988 Jun;41(6):687-90. doi: 10.1136/jcp.41.6.687.
5
Bacteremia due to Mycobacterium avium-intracellulare in the acquired immunodeficiency syndrome.获得性免疫缺陷综合征中鸟分枝杆菌-胞内分枝杆菌所致的菌血症
Ann Intern Med. 1983 Dec;99(6):782-5. doi: 10.7326/0003-4819-99-6-782.
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Primary isolation of Mycobacterium avium complex-serotype 6 on blood agar.在血琼脂上对鸟分枝杆菌复合群血清型6进行初次分离。
J Clin Microbiol. 1976 Sep;4(3):266-9. doi: 10.1128/jcm.4.3.266-269.1976.
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Laboratory diagnosis of mycobacterial infections in patients with acquired immunodeficiency syndrome.获得性免疫缺陷综合征患者分枝杆菌感染的实验室诊断
J Clin Microbiol. 1986 Nov;24(5):708-11. doi: 10.1128/jcm.24.5.708-711.1986.
8
Rapid identification using a specific DNA probe of Mycobacterium avium complex from patients with acquired immunodeficiency syndrome.利用特异性DNA探针从获得性免疫缺陷综合征患者中快速鉴定鸟分枝杆菌复合群。
J Clin Microbiol. 1987 Aug;25(8):1551-2. doi: 10.1128/jcm.25.8.1551-1552.1987.
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Comparison of three methods for recovery of Mycobacterium avium complex from blood specimens.三种从血液标本中回收鸟分枝杆菌复合群方法的比较。
J Clin Microbiol. 1988 Jun;26(6):1225-6. doi: 10.1128/jcm.26.6.1225-1226.1988.
10
Cumulative positivity rates of multiple blood cultures for Mycobacterium avium-intracellulare and Cryptococcus neoformans in patients with the acquired immunodeficiency syndrome.获得性免疫缺陷综合征患者中鸟分枝杆菌-胞内分枝杆菌复合体和新型隐球菌多次血培养的累积阳性率。
Arch Pathol Lab Med. 1990 Sep;114(9):923-5.

引用本文的文献

1
Update on detection of bacteremia and fungemia.菌血症和真菌血症检测的最新进展。
Clin Microbiol Rev. 1997 Jul;10(3):444-65. doi: 10.1128/CMR.10.3.444.
2
Diagnostic utility of bone marrow sampling in HIV positive patients.骨髓采样在HIV阳性患者中的诊断效用。
Genitourin Med. 1997 Apr;73(2):117-21. doi: 10.1136/sti.73.2.117.
3
Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia.使用裂解离心法(隔离器)和放射性测量法(BACTEC)血液培养系统检测分枝杆菌血症。
J Clin Microbiol. 1985 Oct;22(4):543-6. doi: 10.1128/jcm.22.4.543-546.1985.
4
Comparative recoveries of Mycobacterium avium-M. intracellulare from isolator lysis-centrifugation and BACTEC 13A blood culture systems.从隔离器裂解离心和BACTEC 13A血培养系统中回收鸟分枝杆菌-胞内分枝杆菌的比较回收率。
J Clin Microbiol. 1988 Apr;26(4):760-1. doi: 10.1128/jcm.26.4.760-761.1988.
5
Laboratory diagnosis of mycobacterial infections in patients with acquired immunodeficiency syndrome.获得性免疫缺陷综合征患者分枝杆菌感染的实验室诊断
J Clin Microbiol. 1986 Nov;24(5):708-11. doi: 10.1128/jcm.24.5.708-711.1986.
6
AIDS-related mycobacterial disease.艾滋病相关分枝杆菌病
Springer Semin Immunopathol. 1988;10(4):375-91. doi: 10.1007/BF02053847.
7
Mycobacterial disease, immunosuppression, and acquired immunodeficiency syndrome.分枝杆菌病、免疫抑制与获得性免疫缺陷综合征。
Clin Microbiol Rev. 1989 Oct;2(4):360-77. doi: 10.1128/CMR.2.4.360.
8
Diagnosis of Mycobacterium bacteremia in patients with acquired immunodeficiency syndrome by direct examination of blood films.通过直接检查血涂片诊断获得性免疫缺陷综合征患者的分枝杆菌血症
J Clin Microbiol. 1989 Apr;27(4):768-9. doi: 10.1128/jcm.27.4.768-769.1989.

本文引用的文献

1
Increase in Mycobacterium avium complex isolations among patients admitted to a general hospital.综合医院收治患者中鸟分枝杆菌复合群分离株数量增加。
Public Health Rep. 1982 Jan-Feb;97(1):61-5.
2
Radiometric detection of mycobacteria in routine blood cultures.常规血培养中分枝杆菌的放射性检测
J Clin Microbiol. 1980 Sep;12(3):477-8. doi: 10.1128/jcm.12.3.477-478.1980.
3
Optimal time for routine early subculture of blood cultures.血培养常规早期传代的最佳时间。
J Clin Microbiol. 1980 Sep;12(3):445-6. doi: 10.1128/jcm.12.3.445-446.1980.
4
Disseminated Mycobacterium avium-intracellulare infection in homosexual men dying of acquired immunodeficiency.死于获得性免疫缺陷的同性恋男性中的播散性鸟分枝杆菌-胞内分枝杆菌感染。
JAMA. 1982 Dec 10;248(22):2980-2. doi: 10.1001/jama.1982.03330220024029.
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Isolation of Mycobacterium chelonei with the lysis-centrifugation blood culture technique.采用裂解离心血液培养技术分离龟分枝杆菌。
J Clin Microbiol. 1982 Aug;16(2):403-5. doi: 10.1128/jcm.16.2.403-405.1982.
6
Increased suppressor cell activity in a patient with Mycobacterium avium-intracellulare pulmonary disease and hypogammaglobulinemia.鸟分枝杆菌-胞内分枝杆菌肺部疾病合并低丙种球蛋白血症患者抑制细胞活性增加。
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7
Mycobacteremia and the new blood culture systems.分枝杆菌血症与新型血培养系统
Ann Intern Med. 1983 Dec;99(6):786-9. doi: 10.7326/0003-4819-99-6-786.
8
Detection time for blood culture isolates using a biphasic medium.使用双相培养基检测血培养分离株的时间。
Eur J Clin Microbiol. 1983 Feb;2(1):54-5. doi: 10.1007/BF02019926.
9
Retrospective comparison of three blood culture media for the recovery of yeasts from clinical specimens.三种血培养培养基用于从临床标本中分离酵母菌的回顾性比较。
Eur J Clin Microbiol. 1983 Feb;2(1):22-5. doi: 10.1007/BF02019918.
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Disseminated Mycobacterium avium-intracellulare infection in homosexual men with acquired cell-mediated immunodeficiency: a histologic and immunologic study of two cases.
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用于从获得性免疫缺陷综合征患者血液中分离分枝杆菌的新型双相培养系统。

New biphasic culture system for isolation of mycobacteria from blood of patients with acquired immune deficiency syndrome.

作者信息

Berlin O G, Zakowski P, Bruckner D A, Johnson B L

出版信息

J Clin Microbiol. 1984 Sep;20(3):572-4. doi: 10.1128/jcm.20.3.572-574.1984.

DOI:10.1128/jcm.20.3.572-574.1984
PMID:6490837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271373/
Abstract

A new biphasic medium consisting of a 7H11 agar slant and brain heart infusion broth liquid phase has been used for the past 10 months at the University of California at Los Angeles for isolation of Mycobacterium avium complex from blood. In 12 patients whose blood cultures were grown on this medium, the median time for isolation of M. avium complex was 7 days (range 6 to 15 days) compared with 21 to 27 days on a fungal medium and 28 to 38 days on routine blood culture medium. The system has provided a reliable and rapid way to obtain morphology and rapid identification of colonies produced from blood samples of patients with acquired immune deficiency syndrome, who are at high risk for M. avium complex mycobacteremia.

摘要

一种由7H11琼脂斜面和脑心浸液肉汤液相组成的新型双相培养基,在过去10个月里一直被加利福尼亚大学洛杉矶分校用于从血液中分离鸟分枝杆菌复合群。在12例血液培养物在此培养基上生长的患者中,分离出鸟分枝杆菌复合群的中位时间为7天(范围为6至15天),相比之下,在真菌培养基上为21至27天,在常规血液培养基上为28至38天。该系统为从获得性免疫缺陷综合征患者的血样中培养出的菌落获取形态学及快速鉴定提供了一种可靠且快速的方法,这些患者发生鸟分枝杆菌复合群菌血症的风险很高。