Marcusson J, Oreland L, Winblad B
J Neurochem. 1984 Dec;43(6):1699-705. doi: 10.1111/j.1471-4159.1984.tb06098.x.
The effect of age on the binding of [3H]5-hydroxytryptamine [( 3H]5-HT, serotonin) to postmortem human frontal cortex, hippocampus, and putamen from individuals between the ages of 19 and 100 years was studied. One high-affinity binding site was observed in adult brains, with a mean KD of 3.7 nM and 3.2 nM for frontal cortex and hippocampus, respectively, and 9.2 nM for putamen. Decreased binding capacities (Bmax) with age were detected in frontal cortex and hippocampus. In putamen a decrease in affinity was noted. Postmortem storage did not significantly contribute to the age-related changes. No significant sex differences were detected. [3H]5-HT binding was also studied in brains from human neonates. The specific binding was 1.5-3 times lower than in adult frontal cortex and putamen, and Scatchard analysis suggested more than one binding site. In infant hippocampus a single binding site was observed and except for a premature individual, the binding capacity approximated adult values.
研究了年龄对19至100岁个体死后人类额叶皮质、海马体和壳核中[3H]5-羟色胺（[3H]5-HT，血清素）结合的影响。在成人大脑中观察到一个高亲和力结合位点，额叶皮质和海马体的平均解离常数（KD）分别为3.7 nM和3.2 nM，壳核为9.2 nM。在额叶皮质和海马体中检测到结合容量（Bmax）随年龄降低。在壳核中观察到亲和力下降。死后储存对与年龄相关的变化没有显著影响。未检测到显著的性别差异。还研究了人类新生儿大脑中的[3H]5-HT结合。特异性结合比成人额叶皮质和壳核低1.5至3倍，Scatchard分析表明存在多个结合位点。在婴儿海马体中观察到一个单一结合位点，除了一个早产儿外，结合容量接近成人值。
