Gelberman R H, Manske P R, Vande Berg J S, Lesker P A, Akeson W H
J Orthop Res. 1984;2(1):39-48. doi: 10.1002/jor.1100020107.
Flexor tendon healing in four different animal species was explored in a tissue culture system. Ninety percent transverse lacerations were made in 88 tendon segments obtained from rabbits, chickens, dogs, and monkeys. The tendons were removed from culture and studied by light and electron microscopy at intervals of 3, 6, 9, and 12 weeks. A characteristic sequence of repair including epitenon thickening, cellular differentiation, cell migration, and phagocytosis was seen in each of the repaired tendons. The endotenon cells of several animal tendons appeared to be synthesizing collagen. There was a consistent difference in the rate of healing between the four species. The rabbit tendons demonstrated nearly complete closure of the repair site by 12 weeks. A lesser response was seen in the chicken, followed by the dog and monkey. The differences in healing rate appeared to be due to the non-species-specific in vitro culture media. The in vitro flexor tendon culture system is particularly useful in studying the tendon repair responses of various species with the contributions of vascularity and synovial cells excluded.
在组织培养系统中研究了四种不同动物物种的屈肌腱愈合情况。从兔子、鸡、狗和猴子身上获取了88个肌腱节段，制造了90%的横向撕裂伤。每隔3周、6周、9周和12周从培养物中取出肌腱，通过光学显微镜和电子显微镜进行研究。在每一条修复的肌腱中都观察到了包括腱外膜增厚、细胞分化、细胞迁移和吞噬作用在内的特征性修复序列。几种动物肌腱的腱内膜细胞似乎在合成胶原蛋白。这四个物种之间的愈合速度存在一致的差异。兔肌腱在12周时修复部位几乎完全闭合。鸡的反应较小，其次是狗和猴子。愈合速度的差异似乎是由于非物种特异性的体外培养基所致。体外屈肌腱培养系统在研究排除血管和滑膜细胞影响的各种物种的肌腱修复反应方面特别有用。
