Lundblad L, Lundberg J M
Toxicology. 1984 Oct;33(1):1-7. doi: 10.1016/0300-483x(84)90011-8.
Cigarette-smoke exposure in awake guinea-pigs induced reproducible nose wipings with the fore-paws. This behaviour was used as a model to study the mechanisms underlying the irritant effect of cigarette smoke. The nose wipings upon smoke exposure were abolished after local anaesthesia of the nasal mucosa, which indicates that this response was due to activation of nasal protective reflexes. Removal of the particulate phase of the smoke including nicotine using a Cambridge filter only slightly reduced the wiping response, and nicotine-free cigarettes also induced marked irritation. This suggests that irritant chemicals in the vapour phase of the smoke caused this effect. Systemic capsaicin pretreatment, which is known to cause a functional impairment of chemosensitive C-fibre afferents, abolished the nose wiping behaviour seen upon smoke exposure. Local pretreatment of the nasal mucosa with capsaicin also significantly reduced the number of smoke-induced nose wipings. It is concluded that smoke-induced irritation, as indicated by nose wipings, is primarily due to activation of capsaicin-sensitive sensory nerves in the nasal mucosa by vapour-phase components.
清醒的豚鼠暴露于香烟烟雾中会引起前爪反复擦拭鼻子的行为。这种行为被用作模型来研究香烟烟雾刺激作用的潜在机制。对鼻粘膜进行局部麻醉后，暴露于烟雾时的擦鼻行为消失，这表明这种反应是由于鼻保护性反射的激活。使用剑桥滤器去除烟雾的颗粒相（包括尼古丁）只会轻微降低擦拭反应，不含尼古丁的香烟也会引起明显的刺激。这表明烟雾气相中的刺激性化学物质导致了这种效应。已知全身给予辣椒素预处理会导致化学敏感C纤维传入神经功能受损，它消除了暴露于烟雾时出现的擦鼻行为。用辣椒素对鼻粘膜进行局部预处理也显著减少了烟雾引起的擦鼻次数。得出的结论是，如擦鼻所示，烟雾引起的刺激主要是由于气相成分激活了鼻粘膜中对辣椒素敏感的感觉神经。
