Polefka T G, Garrick R A, Redwood W R, Swislocki N I, Chinard F P
Am J Physiol. 1984 Nov;247(5 Pt 1):C350-6. doi: 10.1152/ajpcell.1984.247.5.C350.
A differential centrifugation technique, in which all extracellular water except that intimately associated with the cell (pericellular domain) is removed, has been applied to isolated Novikoff hepatoma cells. The pericellular volumes accessible to albumin, inulin, raffinose, and sucrose were inversely related to the molecular weights of the test solutes. This phenomenon was not detectable in erythrocytes or in fat cells. Selective removal of cell surface components by enzymatic treatment produced proportional changes in the relative volumes of distribution accessible to the solutes. This discrimination in the volume accessible to each of the solutes is analogous to that obtained in gel chromatographic separation and represents, in effect, excluded volumes which are inversely related to solute size. This exclusion is associated with components of the Novikoff cell surface, including the surface coat and the microvilli that cover the Novikoff cell. These structures provide an additional level of discrimination for the Novikoff cell not seen in certain other cell types.
一种差速离心技术已应用于分离的诺维科夫肝癌细胞，该技术可去除所有与细胞紧密相关的细胞外水（细胞周缘区域）之外的细胞外水。白蛋白、菊粉、棉子糖和蔗糖可进入的细胞周缘体积与测试溶质的分子量呈反比关系。这种现象在红细胞或脂肪细胞中无法检测到。通过酶处理选择性去除细胞表面成分会使溶质可进入的相对分布体积产生成比例的变化。每种溶质可进入体积的这种差异类似于凝胶色谱分离中获得的差异，实际上代表了与溶质大小呈反比的排阻体积。这种排阻与诺维科夫细胞表面的成分有关，包括表面被膜和覆盖诺维科夫细胞的微绒毛。这些结构为诺维科夫细胞提供了在某些其他细胞类型中未见的额外一层区分能力。
