Casey M L, Johnston J M, MacDonald P C
Early Hum Dev. 1984 Sep;10(1-2):13-22. doi: 10.1016/0378-3782(84)90107-5.
Human fetal kidney tissue was found to be rich in NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) activity. The enzyme activity was localized primarily in the medullary portion of fetal kidney. Separated cortical and medullary tissue fragments were maintained in organ culture for up to 9 days. The specific activity of PGDH in cortical tissue was low at the commencement of organ culture and remained low throughout the culture period. The specific activity of PGDH in medullary tissue fell precipitously during the first 48 h in organ culture; thereafter, the specific activity of the enzyme increased steadily during the culture period and reached values of up to ten times that in starting tissue by 6-7 days in organ culture. The decline in specific activity of PGDH in medullary tissue during the first 48 h in culture could be prevented by including inhibitors of lysosomal enzyme activity, i.e., chloroquine or ammonium chloride, in the culture medium. We suggest that human fetal kidney tissue explants in organ culture may be an appropriate model for the study of the regulation of PGDH activity. Such an evaluation of the regulation of PGDH is believed to be of importance since PGDH serves to regulate the levels of and, thence, the action of the biologically active prostaglandins.
研究发现，人类胎儿肾脏组织富含烟酰胺腺嘌呤二核苷酸（NAD⁺）依赖性15-羟基前列腺素脱氢酶（PGDH）活性。该酶活性主要定位于胎儿肾脏的髓质部分。将分离出的皮质和髓质组织片段进行器官培养长达9天。在器官培养开始时，皮质组织中PGDH的比活性较低，且在整个培养期间一直保持较低水平。在器官培养的最初48小时内，髓质组织中PGDH的比活性急剧下降；此后，在培养期间该酶的比活性稳步增加，到器官培养6 - 7天时达到起始组织中比活性的十倍之多。在培养基中加入溶酶体酶活性抑制剂，即氯喹或氯化铵，可防止培养最初48小时内髓质组织中PGDH比活性的下降。我们认为，器官培养中的人类胎儿肾脏组织外植体可能是研究PGDH活性调节的合适模型。鉴于PGDH用于调节生物活性前列腺素的水平及作用，因此对PGDH调节的此类评估被认为具有重要意义。
