West P A, Okpokwasili G C, Brayton P R, Grimes D J, Colwell R R
Appl Environ Microbiol. 1984 Nov;48(5):988-93. doi: 10.1128/aem.48.5.988-993.1984.
Phenanthrene-degrading bacteria were isolated from Chesapeake Bay samples by the use of a solid medium which had been overlaid with an ethanol solution of phenanthrene before inoculation. Eighteen representative strains of phenanthrene-degrading bacteria with 21 type and reference bacteria were examined for 123 characteristics representing physiological, biochemical, and nutritional properties. Relationships between strains were computed with several similarity coefficients. The phenogram constructed by unweighted-pair-group arithmetic average linkage and use of the simple Jaccard (SJ) coefficient was used to identify seven phena. Phenanthrene-degrading bacteria were identified as Vibrio parahaemolyticus and Vibrio fluvialis by their clustering with type and reference strains. Several phenanthrene-degrading bacteria resembled Enterobacteriaceae family members, although some Vibrio-like phenanthrene degraders could not be identified.
通过使用一种在接种前已用菲的乙醇溶液覆盖的固体培养基，从切萨皮克湾样本中分离出了菲降解细菌。对18株具有代表性的菲降解细菌菌株以及21株模式菌株和参考菌株进行了检测，以分析代表生理、生化和营养特性的123个特征。用几种相似系数计算菌株之间的关系。通过不加权配对组算术平均连锁法和使用简单杰卡德（SJ）系数构建的系统树图用于识别七个类群。通过与模式菌株和参考菌株聚类，菲降解细菌被鉴定为副溶血性弧菌和河流弧菌。尽管一些类似弧菌的菲降解菌无法鉴定，但几种菲降解细菌与肠杆菌科成员相似。
