Gilbard J P, Carter J B, Sang D N, Refojo M F, Hanninen L A, Kenyon K R
Ophthalmology. 1984 Oct;91(10):1205-12. doi: 10.1016/s0161-6420(84)34163-x.
The morphologic effect of hyperosmolarity, equivalent to that seen in the tear film of patients with keratoconjunctivitis sicca (KCS), on rabbit corneal epithelium in vitro and in vivo was studied. In the in vitro studies, corneal epithelium was grown in explant cultures. Control tissue was cultured in a 307 mOsm/L medium. Epithelium cultured in the 333, 361 and 363 mOsm/L media showed decreased intercellular connections, blunting and loss of microplicae, disruptions in cell membranes and cellular swelling with decreased cytoplasmic density. In in vivo studies, corneas bathed in balanced salt solutions (BSS) concentrated to 330, 360, or 407 mOsm/L showed increased cell desquamation, and the cell changes observed at similar osmolarities in the in vitro studies. The tear film osmolarities observed in KCS are sufficient to cause the corneal epithelial changes seen in patients with this disease.
研究了与干燥性角结膜炎（KCS）患者泪膜中所见相同的高渗状态对兔角膜上皮细胞在体外和体内的形态学影响。在体外研究中，角膜上皮细胞在外植体培养物中生长。对照组织在307 mOsm/L的培养基中培养。在333、361和363 mOsm/L培养基中培养的上皮细胞显示细胞间连接减少、微褶变钝和消失、细胞膜破坏以及细胞肿胀，细胞质密度降低。在体内研究中，浸泡在浓缩至330、360或407 mOsm/L的平衡盐溶液（BSS）中的角膜显示细胞脱屑增加，以及在体外研究中在相似渗透压下观察到的细胞变化。KCS患者观察到的泪膜渗透压足以引起该疾病患者角膜上皮的变化。
