Hirsch R S, McClure J, Vernon-Roberts B
Aust J Exp Biol Med Sci. 1984 Dec;62 ( Pt 6):727-42. doi: 10.1038/icb.1984.69.
Granulation tissue was allowed to grow into polyurethane sponges implanted subcutaneously in rats for 14 days prior to injection of saline (control) or lead acetate (PbAc) into the sponge centres. The reaction was studied for 21 days post-injection utilising sponge dry weight gain, histology and electron microscopy. Five hours after PbAc injection, calcification was demonstrable within the central tissue-free portion of the sponges. Calcification in granulation tissue was associated with collagen fibres and elicited a macrophage and multinucleated giant cell reaction. Mast cells were not found within sponges. Hydroxyapatite deposition was associated with an increase in sponge weight compared with controls. The injection of PbAc elicited calcification in granulation tissue in the same time scale reported for calcergy in mature connective tissue and the phenomenon of calcergy does not require the presence of collagen fibres or mast cells in its early stages.
在将生理盐水（对照）或醋酸铅（PbAc）注入海绵中心之前，先让肉芽组织在皮下植入大鼠体内的聚氨酯海绵中生长14天。注射后21天，利用海绵干重增加、组织学和电子显微镜对反应进行研究。注射PbAc 5小时后，在海绵的中央无组织部分可证实有钙化。肉芽组织中的钙化与胶原纤维有关，并引发巨噬细胞和多核巨细胞反应。在海绵中未发现肥大细胞。与对照相比，羟基磷灰石沉积与海绵重量增加有关。PbAc的注射在与成熟结缔组织中钙能反应所报道的相同时间范围内引发肉芽组织钙化，并且钙能现象在其早期阶段不需要胶原纤维或肥大细胞的存在。
