LaThangue N B
EMBO J. 1984 Aug;3(8):1871-9. doi: 10.1002/j.1460-2075.1984.tb02061.x.
A monoclonal antibody reacts with a polypeptide of 68 000 mol. wt. (p68) that accumulates to high levels during heat shock. The intracellular distribution of this antigen in normal and heat-shocked cells has been studied. It is a major component of non-stressed cells, where it is located predominantly in the cytoplasm, but also occurs in the nucleus. The nuclear accumulation is growth regulated, in that exponentially growing cells have strong nuclear immunofluorescence and confluent cells little. It is concentrated at the leading edge of motile fibroblasts and co-distributes with actin-containing microfilaments. Heat shock causes cytoplasmic and nuclear accumulation and there is new deposition in the periphery of cells. In normal cells the antigen in the nucleus is located in the nuclear lamina and matrix which increases during heat shock. The distribution of this molecule and the structures with which it interacts suggests that it is important in mediating the effects of heat shock.
一种单克隆抗体可与分子量为68000道尔顿的多肽（p68）发生反应，该多肽在热休克期间会大量积累。已对这种抗原在正常细胞和热休克细胞中的细胞内分布进行了研究。它是未受应激细胞的主要成分，主要位于细胞质中，但也存在于细胞核中。核积累受生长调节，即指数生长的细胞具有强烈的核免疫荧光，而汇合细胞则很少。它集中在运动性成纤维细胞的前缘，并与含肌动蛋白的微丝共同分布。热休克导致细胞质和细胞核积累，并且在细胞周边有新的沉积。在正常细胞中，细胞核中的抗原位于核纤层和核基质中，热休克期间会增加。这种分子的分布及其相互作用的结构表明，它在介导热休克的影响方面很重要。
