Krawczyk Z, Wiśniewski J, Biesiada E
Mol Biol Rep. 1987;12(1):27-34. doi: 10.1007/BF00580647.
The expression pattern of major heat shock related genes (hsp70 gene family) in various organs of mouse and rat was investigated using Northern blot analysis. Heat shock gene related transcripts were detected in total RNA by hybridization with cloned mouse hsp70 gene sequences. Cells of various organs of intact mouse and rat constitutively synthesize a 2.2 kb and a 2.5 kb RNA. Exceptionally high levels of the 2.5 kb RNA, 50-250 fold higher than in other tissues are found in testis of both rodents. The 2.5 kb RNA hybridizes strongly to an extended region of mouse hsp70 gene; it also hybridizes poorly to the Drosophila hsp70 gene. The data suggest that the 2.5 kb RNA is transcribed from a hsp70-related gene in mouse and rat.
利用Northern印迹分析，研究了小鼠和大鼠各器官中主要热休克相关基因（hsp70基因家族）的表达模式。通过与克隆的小鼠hsp70基因序列杂交，在总RNA中检测到热休克基因相关转录本。完整小鼠和大鼠各器官的细胞组成性地合成2.2 kb和2.5 kb的RNA。在两种啮齿动物的睾丸中发现2.5 kb RNA的水平极高，比其他组织高50 - 250倍。2.5 kb RNA与小鼠hsp70基因的一个延伸区域强烈杂交；它与果蝇hsp70基因的杂交效果也很差。数据表明，2.5 kb RNA是从小鼠和大鼠中一个与hsp70相关的基因转录而来的。
