冬季呕吐病中的病毒样颗粒

Virus-like particles in winter vomiting disease.

作者信息

Appleton H, Buckley M, Thom B T, Cotton J L, Henderson S

出版信息

Lancet. 1977 Feb 19;1(8008):409-11. doi: 10.1016/s0140-6736(77)92614-9.

Abstract

In an outbreak of winter vomiting disease affecting both pupils and staff in a primary school, virus-like particles were found in 7 out of 8 faecal specimens examined by electron microscopy. The particles measured 26 nm in diameter and had a buoyant density of 1-38--1-40 g/cm3 in caesium chloride. They could not be cultured in tissue-culture or organ-culture. In immune electron microscopy tests the particles appeared to differ antigenically from the Norwalk and Hawaii agents. Two out of three patients examined more than one month after their illness were still excreting the particles.

摘要

在一所小学发生的一起影响学生和教职工的冬季呕吐病疫情中，通过电子显微镜检查的8份粪便标本中有7份发现了病毒样颗粒。这些颗粒直径为26纳米，在氯化铯中的浮力密度为1.38至1.40克/立方厘米。它们无法在组织培养或器官培养中培养。在免疫电子显微镜测试中，这些颗粒在抗原性上似乎与诺沃克病毒和夏威夷病毒不同。三名患病一个多月后接受检查的患者中有两名仍在排出这些颗粒。