Blazar B A, Vanky F, Klein E
Cancer Immunol Immunother. 1984;18(3):174-8. doi: 10.1007/BF00205508.
Tumor and lymphoid cell components from primary mammary adenocarcinomas of C3H/He mice were isolated simultaneously by velocity gradients. Viable tumor cells were obtained in sufficient numbers to test their in vivo and in vitro growth. Isolated tumor cells grew in 97% of inoculated syngeneic animals. In six assays with different tumors the effects of tumor-associated lymphoid cells (TAL) on in vivo tumor growth varied, enhancing in three and delaying in two experiments. Isolated tumor cells from animals with enhancing TAL grew faster in nonirradiated mice, whereas tumor cells from animals with inhibitory TAL grew better in irradiated animals. Isolated tumor cells also proliferated in cell culture, where they averaged 35% primary plating efficiency. Separated tumor cells were used in short-term 51Cr-release assays with TAL, tumor-bearer lymph node and spleen effectors. Cytotoxicity was detected in only five of 25 assays. In no case was there killing by lymphocyte populations from normal animals. In the present report we describe a technique for the isolation of viable tumor and lymphoid cells from murine adenocarcinomas that allows study of interactions between these populations from the original tumor-bearing host.
通过速度梯度同时从C3H/He小鼠的原发性乳腺腺癌中分离肿瘤和淋巴细胞成分。获得了足够数量的活肿瘤细胞以测试其体内和体外生长情况。分离出的肿瘤细胞在97%的接种同基因动物中生长。在对不同肿瘤进行的六项试验中，肿瘤相关淋巴细胞（TAL）对体内肿瘤生长的影响各不相同，在三项实验中起增强作用，在两项实验中起延迟作用。来自具有增强TAL的动物的分离肿瘤细胞在未受照射的小鼠中生长更快，而来自具有抑制性TAL的动物的肿瘤细胞在受照射的动物中生长更好。分离出的肿瘤细胞也能在细胞培养中增殖，其原代接种效率平均为35%。将分离出的肿瘤细胞用于与TAL、荷瘤动物淋巴结和脾效应细胞进行的短期51Cr释放试验。在25项试验中仅在5项试验中检测到细胞毒性。正常动物的淋巴细胞群体在任何情况下均未产生杀伤作用。在本报告中，我们描述了一种从鼠腺癌中分离活肿瘤细胞和淋巴细胞的技术，该技术能够研究来自原始荷瘤宿主的这些细胞群体之间的相互作用。
