Wolinsky H, Capron L, Goldfischer S, Capron F, Coltoff-Schiller B, Kasak L E
Circ Res. 1978 Jun;42(6):831-9. doi: 10.1161/01.res.42.6.831.
Hypertension is an important risk factor for atherosclerosis and often occurs in association with diabetes mellitus. Specific activities of hydrolases in homogenates of aortas from rats with renal-clip hypertension, normotension following a period of hypertension, and hypertension combined with streptozotocin-induced diabetes mellitus were measured. Enzymes included: neutral alpha-glucosidase, and lysosomal N-acetyl-beta-glucosaminidase, beta-galactosidase, cathepsin C, acid alpha-glucosidase, and acid cholesteryl esterase. After 6 or 12 weeks of hypertension, specific activities of all enzymes measured were significantly increased, levels ranging from 24% above normal for cathepsin C to 351% above normal for N-acetyl-beta-glucosaminidase. Six weeks of normotension following 6 weeks of hypertension resulted in restoration to normal of four of the six enzyme activities; the remaining two enzymes were significantly below normal levels. Combined hypertension and diabetes mellitus showed smooth muscle cell levels of four of the five hydrolases measured to be significantly lower than those present with hypertension alone. In every instance, histochemical studies of aortas showed acid phosphatase and N-acetyl-beta-glucosaminidase activities which corresponded to the biochemical findings. These findings indicate profound and discrete effects of two clinical risk factors on vascular smooth muscle cell lysosomes.
高血压是动脉粥样硬化的重要危险因素，且常与糖尿病并发。对肾动脉夹闭型高血压大鼠、高血压一段时间后恢复正常血压的大鼠以及高血压合并链脲佐菌素诱导的糖尿病大鼠的主动脉匀浆中的水解酶的特定活性进行了测定。所测酶包括：中性α-葡萄糖苷酶、溶酶体N-乙酰-β-氨基葡萄糖苷酶、β-半乳糖苷酶、组织蛋白酶C、酸性α-葡萄糖苷酶和酸性胆固醇酯酶。高血压6周或12周后，所测所有酶的特定活性均显著增加，增幅从组织蛋白酶C高于正常水平24%到N-乙酰-β-氨基葡萄糖苷酶高于正常水平351%不等。高血压6周后恢复正常血压6周，六种酶活性中的四种恢复到正常水平；其余两种酶显著低于正常水平。高血压合并糖尿病时，所测五种水解酶中的四种平滑肌细胞水平显著低于单纯高血压时。在每种情况下，主动脉的组织化学研究显示酸性磷酸酶和N-乙酰-β-氨基葡萄糖苷酶活性与生化研究结果一致。这些发现表明两种临床危险因素对血管平滑肌细胞溶酶体有深刻而独特的影响。
