Vollmers H P, Birchmeier W
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3729-33. doi: 10.1073/pnas.80.12.3729.
Seven monoclonal antibodies against mouse B 16 melanoma cells (produced in syngeneic C57BL/6 mice) were selected that blocked the adhesion of melanoma cells to tissue culture dishes. These antibodies were found to be directed against antigens on the surface of mouse B 16 melanoma cells but not on normal mouse cells such as 3T3 fibroblasts. Similarly, the antigens could not be detected in normal mouse tissues (e.g., lung, kidney, liver) but were found in lungs colonized by B 16 melanoma cells. Significantly, three of these antibodies virtually abolished lung colonization of highly invasive B 16 sublines injected into the animals' bloodstream. They exerted their effect both when preabsorbed by the melanoma cell in vitro and when delivered to the animals prior to the tumor cells. It is suggested that monoclonal antibodies might be a promising tool for preventing metastasis.
筛选出七种针对小鼠B16黑色素瘤细胞（由同基因C57BL/6小鼠产生）的单克隆抗体，它们能阻断黑色素瘤细胞与组织培养皿的黏附。发现这些抗体针对的是小鼠B16黑色素瘤细胞表面的抗原，而非正常小鼠细胞如3T3成纤维细胞表面的抗原。同样，在正常小鼠组织（如肺、肾、肝）中检测不到这些抗原，但在被B16黑色素瘤细胞定植的肺中能发现。值得注意的是，其中三种抗体几乎完全消除了注入动物血流中的高侵袭性B16亚系在肺部的定植。无论是在体外被黑色素瘤细胞预先吸附，还是在肿瘤细胞之前给动物注射，它们都能发挥作用。有人提出单克隆抗体可能是预防转移的一种有前景的工具。
