Gambke C, Signer E, Moroni C
Nature. 1984;307(5950):476-8. doi: 10.1038/307476a0.
Human tumour cell lines of various histological origin contain genes that can transform NIH 3T3 cells in culture. Most frequently the gene is an activated K-ras gene, more rarely an activated H-ras gene, and sometimes the recently discovered N-ras. Other transforming genes, distinct from ras, have been found in B- and T-cell leukaemias. Since most of the transforming genes have been identified in cell lines, it is still unclear at what stage the genes become activated. We have therefore initiated a study to determine if the presence of a transforming gene correlates with the clinical course of a malignant disease. Here we demonstrate the presence of a transforming N-ras gene in bone marrow cells from a patient with acute myeloblastic leukaemia at the outbreak of the acute disease phase. Fibroblast DNA from the same patient was not transforming. In contrast to HL-60 cells, no alteration of the myc gene was detected.
各种组织学来源的人类肿瘤细胞系含有能够在培养中转化NIH 3T3细胞的基因。最常见的基因是激活的K-ras基因，较少见的是激活的H-ras基因，有时是最近发现的N-ras基因。在B细胞和T细胞白血病中发现了其他不同于ras的转化基因。由于大多数转化基因是在细胞系中鉴定出来的，因此这些基因在哪个阶段被激活仍不清楚。因此，我们启动了一项研究，以确定转化基因的存在是否与恶性疾病的临床病程相关。在此我们证明，在一名急性髓细胞白血病患者急性疾病期发作时的骨髓细胞中存在一个转化性N-ras基因。来自同一患者成纤维细胞的DNA没有转化能力。与HL-60细胞不同，未检测到myc基因的改变。
