急性髓细胞白血病中N-ras基因通过第一个外显子的体细胞突变而激活。
Activation of an N-ras gene in acute myeloblastic leukemia through somatic mutation in the first exon.
作者信息
Gambke C, Hall A, Moroni C
出版信息
Proc Natl Acad Sci U S A. 1985 Feb;82(3):879-82. doi: 10.1073/pnas.82.3.879.
Abstract
A transforming N-ras gene has been cloned from acute myeloblastic leukemia bone marrow cells, in parallel with the N-ras gene derived from fibroblasts of the same patient. N-ras derived from fibroblasts lacked focus-forming activity in NIH/3T3 cells, indicating that gene activation in the leukemia cells must have occurred by a somatic event. Construction of chimeric molecules between the transforming and the normal N-ras genes and subsequent biological and sequence analysis of these constructs revealed that the transforming gene was altered by a point mutation changing amino acid 12 of the N-ras protein from glycine to aspartic acid.
摘要
已从急性髓性白血病骨髓细胞中克隆出一个具有转化能力的N-ras基因，同时还克隆了来自同一名患者成纤维细胞的N-ras基因。源自成纤维细胞的N-ras基因在NIH/3T3细胞中缺乏集落形成活性，这表明白血病细胞中的基因激活必定是由体细胞事件引起的。构建转化型和正常N-ras基因之间的嵌合分子，并对这些构建体进行后续的生物学和序列分析，结果显示转化基因发生了点突变，使N-ras蛋白的第12位氨基酸由甘氨酸变为天冬氨酸。
相似文献
1
Activation of an N-ras gene in acute myeloblastic leukemia through somatic mutation in the first exon.急性髓细胞白血病中N-ras基因通过第一个外显子的体细胞突变而激活。
Proc Natl Acad Sci U S A. 1985 Feb;82(3):879-82. doi: 10.1073/pnas.82.3.879.
2
N-ras gene activation in the RD human rhabdomyosarcoma cell line.RD人横纹肌肉瘤细胞系中的N-ras基因激活
Int J Cancer. 1985 May 15;35(5):647-52. doi: 10.1002/ijc.2910350513.
3
Activation of N-ras gene in bone marrow cells from a patient with acute myeloblastic leukaemia.一名急性髓细胞白血病患者骨髓细胞中N-ras基因的激活。
Nature. 1984;307(5950):476-8. doi: 10.1038/307476a0.
4
Activation of a human c-K-ras oncogene.人类c-K-ras癌基因的激活。
Nucleic Acids Res. 1984 Dec 11;12(23):8873-85. doi: 10.1093/nar/12.23.8873.
5
Somatic N-ras oncogene activation in a patient with acute myeloblastic leukemia.一名急性髓细胞白血病患者的体细胞N-ras癌基因激活
Haematol Blood Transfus. 1985;29:159-62. doi: 10.1007/978-3-642-70385-0_35.
6
Activation mechanism of the N-ras oncogene in human leukemias detected by synthetic oligonucleotide probes.用合成寡核苷酸探针检测人类白血病中N-ras癌基因的激活机制。
Biochem Biophys Res Commun. 1987 Sep 15;147(2):870-5. doi: 10.1016/0006-291x(87)91010-2.
7
Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype.人类造血肿瘤的转化基因：频繁检测到与ras相关的癌基因，其激活似乎与肿瘤表型无关。
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4926-30. doi: 10.1073/pnas.80.16.4926.
8
Isolation of transforming sequences of two human lung carcinomas: structural and functional analysis of the activated c-K-ras oncogenes.两例人肺癌转化序列的分离：活化的c-K-ras癌基因的结构与功能分析
Proc Natl Acad Sci U S A. 1984 Jan;81(1):71-5. doi: 10.1073/pnas.81.1.71.
9
Transforming genes in human leukemia cells.人类白血病细胞中的转化基因。
Blood. 1985 Dec;66(6):1371-8.
10
Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080.人纤维肉瘤细胞系HT1080中N-ras基因的激活机制。
EMBO J. 1984 Jun;3(6):1321-6. doi: 10.1002/j.1460-2075.1984.tb01970.x.
引用本文的文献
1
Activated N-Ras signaling regulates arterial-venous specification in zebrafish.激活的 N-Ras 信号调节斑马鱼的动静脉特化。
J Hematol Oncol. 2013 May 12;6:34. doi: 10.1186/1756-8722-6-34.
2
Ras oncogenes: split personalities.Ras癌基因：具有双重特性。
Nat Rev Mol Cell Biol. 2008 Jul;9(7):517-31. doi: 10.1038/nrm2438.
3
Activation of proto-oncogenes in human leukemias.人类白血病中原癌基因的激活。
Blut. 1985 Aug;51(2):63-71. doi: 10.1007/BF00320114.
4
Biochemical and biological properties of the human N-ras p21 protein.人N-ras p21蛋白的生化及生物学特性
Mol Cell Biol. 1987 Jan;7(1):541-4. doi: 10.1128/mcb.7.1.541-544.1987.
5
Human cancer and cellular oncogenes.人类癌症与细胞癌基因。
Biochem J. 1987 Apr 15;243(2):313-27. doi: 10.1042/bj2430313.
6
Transformation of quail embryo fibroblasts by a retrovirus carrying a normal human c-myc gene.携带正常人c-myc基因的逆转录病毒对鹌鹑胚胎成纤维细胞的转化
EMBO J. 1986 Jul;5(7):1529-33. doi: 10.1002/j.1460-2075.1986.tb04393.x.
7
Oncogenicity of human N-ras oncogene and proto-oncogene introduced into retroviral vectors.导入逆转录病毒载体的人N-ras癌基因和原癌基因的致癌性。
J Virol. 1989 Sep;63(9):3944-53. doi: 10.1128/JVI.63.9.3944-3953.1989.
8
A v-H-ras-dependent hemopoietic tumor model involving progression from a clonal stage of transformation competence to autocrine interleukin 3 production.一种依赖v-H-ras的造血肿瘤模型，涉及从转化能力的克隆阶段进展到自分泌白细胞介素3的产生。
Mol Cell Biol. 1989 Mar;9(3):1183-90. doi: 10.1128/mcb.9.3.1183-1190.1989.
本文引用的文献
1
Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.T24膀胱癌转化基因的激活与单个氨基酸变化有关。
Nature. 1982 Dec 23;300(5894):762-5. doi: 10.1038/300762a0.
2
Oncogenes in solid human tumours.人类实体瘤中的癌基因。
Nature. 1982 Dec 9;300(5892):539-42. doi: 10.1038/300539a0.
3
A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.一个点突变导致了T24人膀胱癌癌基因获得转化特性。
Nature. 1982 Nov 11;300(5888):149-52. doi: 10.1038/300149a0.
4
Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.从T24膀胱癌细胞中分离出一种人类转化基因并进行初步鉴定。
Nature. 1982 Apr 1;296(5856):404-9. doi: 10.1038/296404a0.
5
Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.c-bas/has人类原癌基因内的替代点突变导致转化特性的获得。
Nature. 1983 Jun 30;303(5920):775-9. doi: 10.1038/303775a0.
6
Predicted nucleotide-binding properties of p21 protein and its cancer-associated variant.p21蛋白及其癌症相关变体的预测核苷酸结合特性。
Nature. 1983 Apr 28;302(5911):842-4. doi: 10.1038/302842a0.
7
Transforming ras genes from human melanoma: a manifestation of tumour heterogeneity?来自人类黑色素瘤的转化型ras基因：肿瘤异质性的一种表现？
Nature. 1984;308(5954):69-72. doi: 10.1038/308069a0.
8
Somatic activation of rasK gene in a human ovarian carcinoma.人类卵巢癌中rasK基因的体细胞激活。
Science. 1984 Feb 17;223(4637):698-701. doi: 10.1126/science.6695178.
9
Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient.K-ras癌基因在肺癌中发生恶性激活，但在同一患者的正常组织中未发生。
Science. 1984 Feb 17;223(4637):661-4. doi: 10.1126/science.6695174.
10
The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors.HL-60转化序列：一种与造血肿瘤中改变的myc基因共存的ras癌基因。
Cell. 1983 Jul;33(3):749-57. doi: 10.1016/0092-8674(83)90017-x.
Zotero 插件