Gambke C, Hall A, Moroni C
Proc Natl Acad Sci U S A. 1985 Feb;82(3):879-82. doi: 10.1073/pnas.82.3.879.
A transforming N-ras gene has been cloned from acute myeloblastic leukemia bone marrow cells, in parallel with the N-ras gene derived from fibroblasts of the same patient. N-ras derived from fibroblasts lacked focus-forming activity in NIH/3T3 cells, indicating that gene activation in the leukemia cells must have occurred by a somatic event. Construction of chimeric molecules between the transforming and the normal N-ras genes and subsequent biological and sequence analysis of these constructs revealed that the transforming gene was altered by a point mutation changing amino acid 12 of the N-ras protein from glycine to aspartic acid.
已从急性髓性白血病骨髓细胞中克隆出一个具有转化能力的N-ras基因，同时还克隆了来自同一名患者成纤维细胞的N-ras基因。源自成纤维细胞的N-ras基因在NIH/3T3细胞中缺乏集落形成活性，这表明白血病细胞中的基因激活必定是由体细胞事件引起的。构建转化型和正常N-ras基因之间的嵌合分子，并对这些构建体进行后续的生物学和序列分析，结果显示转化基因发生了点突变，使N-ras蛋白的第12位氨基酸由甘氨酸变为天冬氨酸。
