Aukrust L, Apold J, Elsayed S, Aas K
Int Arch Allergy Appl Immunol. 1978;57(3):253-62. doi: 10.1159/000232110.
A standard reference pattern in crossed immunoelectrophoresis (CIE) of the parvalbumin fraction of a cod white muscle extract was established. Crossed line immunoelectrophoresis (CLIE), tandem CIE and crossed radioimmunoelectrophoresis (CRIE) were used to identify the major allergens, thereby establishing a basis for future work employing quantitative immunoelectrophoretic techniques in the study of codfish allergens. Rocket immunoelectrophoresis and rocket-line immunoelectrophoresis were used to demonstrate antigenic determinants on polypeptide fragments of the major allergen, Allergen M. The results show the usefulness of these techniques in studies of antigenic determinants on non-precipitating polypeptides. CRIE with 8 patients's sera showed radiostaining indicating IgE binding corresponding to at least 7 precipitates in the crude extract CIE preparations. The purified DS 22 fraction was shown to contain 2 CRIE-positive (IgE-binding) precipitates. When using Allergen M, these precipitates were also demonstrated, one of them in trace amounts only.
建立了鳕鱼白肌提取物中副肌球蛋白部分的交叉免疫电泳（CIE）标准参考模式。采用交叉线免疫电泳（CLIE）、串联CIE和交叉放射免疫电泳（CRIE）来鉴定主要过敏原，从而为今后在鳕鱼过敏原研究中采用定量免疫电泳技术奠定基础。用火箭免疫电泳和火箭线免疫电泳来证明主要过敏原变应原M多肽片段上的抗原决定簇。结果表明这些技术在研究非沉淀性多肽上的抗原决定簇方面是有用的。用8名患者血清进行的CRIE显示放射性染色，表明IgE结合对应于粗提取物CIE制剂中至少7种沉淀物。纯化的DS 22部分显示含有2种CRIE阳性（IgE结合）沉淀物。当使用变应原M时，也证明了这些沉淀物，其中一种仅为痕量。
