Souyri M, Fleissner E
Proc Natl Acad Sci U S A. 1983 Nov;80(21):6676-9. doi: 10.1073/pnas.80.21.6676.
DNA from human T-cell leukemia cell lines was tested for focus-inducing activity on cultures of NIH 3T3 cells. Three leukemias yielded DNA active in this assay; restriction enzyme sensitivity of this activity indicated that similar, relatively large DNA sequences were involved. Southern blot analysis revealed conserved size classes of restriction fragments containing human repetitive (Alu) sequences in serially transfected foci derived from the active DNAs. Similar blot hybridizations with a probe specific for the human N-ras oncogene detected a 9-kilobase EcoRI fragment in all cases. DNA containing this fragment from one of the leukemias, molecularly cloned in bacteriophage lambda, displayed highly amplified focus-inducing activity in transfection assays. Thus, the N-ras oncogene appears to be active in these three human leukemias of T-cell origin.
对来自人T细胞白血病细胞系的DNA进行检测，以观察其对NIH 3T3细胞培养物的集落诱导活性。三种白血病的DNA在此检测中表现出活性；该活性对限制酶的敏感性表明涉及相似的、相对较大的DNA序列。Southern印迹分析显示，在源自活性DNA的连续转染集落中，含有人类重复（Alu）序列的限制片段存在保守的大小类别。用针对人N-ras癌基因的特异性探针进行的类似印迹杂交在所有情况下均检测到一个9千碱基的EcoRI片段。从其中一种白血病中获得的含有该片段的DNA，经分子克隆到噬菌体λ中后，在转染试验中表现出高度扩增的集落诱导活性。因此，N-ras癌基因在这三种T细胞起源的人类白血病中似乎是有活性的。
