用于检测抗b型流感嗜血杆菌荚膜抗体的酶联免疫吸附测定：与放射免疫测定的比较
Enzyme-linked immunosorbent assay for detection of capsular antibodies against Haemophilus influenzae type b: comparison with radioimmunoassay.
作者信息
Kaplan S L, Mason E O, Johnson G, Broughton R A, Hurley D, Parke J C
出版信息
J Clin Microbiol. 1983 Nov;18(5):1201-4. doi: 10.1128/jcm.18.5.1201-1204.1983.
Abstract
An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration determined by radioimmunoassay. The ELISA technique will be useful for further investigations of host response to infections due to H. influenzae type b but is not intended to be used as a serological method for documenting H. influenzae type b infections.
摘要
本文描述了一种用于检测抗 b 型流感嗜血杆菌荚膜抗体的酶联免疫吸附测定（ELISA）方法。在将多聚核糖磷酸吸附到微量滴定板之前，先将其与聚-L-赖氨酸共价结合。ELISA 检测的免疫球蛋白 G 和免疫球蛋白 M 抗多聚核糖磷酸抗体滴度与通过放射免疫测定法测定的总抗多聚核糖磷酸抗体浓度相当。ELISA 技术将有助于进一步研究宿主对 b 型流感嗜血杆菌感染的反应，但并非旨在用作记录 b 型流感嗜血杆菌感染的血清学方法。
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本文引用的文献
1
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Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测到的针对肺炎球菌多糖的免疫球蛋白G和M抗体。
Infect Immun. 1980 Feb;27(2):411-7. doi: 10.1128/iai.27.2.411-417.1980.
3
Enzyme-linked immunosorbent assay for detection of antibodies against Streptococcus pneumoniae capsular polysaccharides.用于检测抗肺炎链球菌荚膜多糖抗体的酶联免疫吸附测定。
J Clin Microbiol. 1980 Feb;11(2):198-9. doi: 10.1128/jcm.11.2.198-199.1980.
4
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5
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6
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8
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9
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