Hoffman P A, Granade H R, McMillan J P
Toxicon. 1983;21(3):363-9. doi: 10.1016/0041-0101(83)90092-2.
Chromatographically purified ciguatoxin from toxic blackfin snapper (Lutjanus buccanella) obtained from an impounded catch in the U.S. Virgin Islands was used together with a refined mouse bioassay to construct a dose-response curve. The LD50 was calculated to be 87 mg/kg and the range of ciguatoxin doses between 0% and 100% lethality was narrow (62.5-112.5 mg/kg). Symptoms appeared in a rather stereotypic pattern with the most frequent being lowered rectal temperature and reduced locomotor activity and reflexes. Diarrhea and cyanosis were common. Breathing difficulties and convulsions occurred more often at higher doses. Approximately one-half of the animals which succumbed to ciguatoxin showed convulsions at some time, often just prior to death. The mouse bioassay described permits a reproducible and moderately sensitive detection of ciguatoxicity and would, if uniformly adopted, facilitate a comparison among future reports on ciguatera research.
从美属维尔京群岛一处扣押渔获物中获取的有毒黑鳍笛鲷（Lutjanus buccanella）经色谱法纯化得到的雪卡毒素，与改进的小鼠生物测定法一起用于构建剂量反应曲线。计算得出半数致死剂量（LD50）为87毫克/千克，雪卡毒素导致0%至100%致死率的剂量范围较窄（62.5 - 112.5毫克/千克）。症状呈现出相当典型的模式，最常见的是直肠温度降低、运动活动和反射减弱。腹泻和发绀很常见。高剂量时更常出现呼吸困难和惊厥。死于雪卡毒素的动物中约有一半在某个时候出现惊厥，通常就在死亡前。所描述的小鼠生物测定法能够对雪卡毒性进行可重复且适度敏感的检测，若统一采用，将有助于未来雪卡毒素研究报告之间的比较。
