Gentry R T, Rappaport M S, Dole V P
Physiol Behav. 1983 Oct;31(4):529-32. doi: 10.1016/0031-9384(83)90077-x.
A technique permitting the serial determination of plasma ethanol concentration (PEC) in mice is described. Samples of blood, 5 microliters in volume, are drawn from the infraorbital plexus. It was demonstrated that this source reflects the rapid dynamics of absorption and clearance after an intraperitoneal injection of ethanol. Samples taken from the tail showed a greatly delayed response and required 30 min to equilibrate with samples taken from the infraorbital plexus. The concentrations of ethanol were determined by gas chromatography. A precolumn removed the nonvolatile constituents thus permitting the injection of plasma directly into the instrument. The ratio of ethanol concentrations in red blood cells to plasma was found to be 0.62. This permitted the estimation of concentration of ethanol in whole blood: (1-0.38 H) X PEC; where H = the hematocrit.
本文描述了一种用于连续测定小鼠血浆乙醇浓度（PEC）的技术。从眶下丛抽取5微升体积的血液样本。结果表明，该采血部位能反映腹腔注射乙醇后吸收和清除的快速动态变化。从尾部采集的样本显示反应大大延迟，且需要30分钟才能与从眶下丛采集的样本达到平衡。乙醇浓度通过气相色谱法测定。预柱去除了非挥发性成分，从而允许将血浆直接注入仪器。发现红细胞与血浆中乙醇浓度之比为0.62。由此可估算全血中乙醇浓度：（1 - 0.38H）×PEC；其中H = 血细胞比容。
