Ryan R W, Kwasnik I, Tilton R C
Diagn Microbiol Infect Dis. 1983 Mar;1(1):59-63. doi: 10.1016/0732-8893(83)90033-0.
An improved counterimmunoelectrophoresis (CIE) procedure for detection of Clostridium difficile antigen is described. Commercially available antiserum to C. difficile toxin was absorbed with whole cells of C. difficile. CIE (absorbed) was 100% sensitive and 77.5% specific when compared to the tissue culture toxin assay. Instances are noted in which the CIE (absorbed) and/or bacterial culture was positive and the tissue culture assay was negative.
本文描述了一种用于检测艰难梭菌抗原的改良对流免疫电泳（CIE）方法。用艰难梭菌全细胞吸收市售的抗艰难梭菌毒素抗血清。与组织培养毒素检测相比，吸收后的CIE敏感性为100%，特异性为77.5%。文中指出了一些情况，即吸收后的CIE和/或细菌培养呈阳性而组织培养检测呈阴性。
