人单核细胞的体外分化。在玻璃上培养的单核细胞对肿瘤细胞具有细胞毒性，但在胶原蛋白上培养的单核细胞则不具有。
In vitro differentiation of human monocytes. Monocytes cultured on glass are cytotoxic to tumor cells but monocytes cultured on collagen are not.
作者信息
Kaplan G
出版信息
J Exp Med. 1983 Jun 1;157(6):2061-72. doi: 10.1084/jem.157.6.2061.
Abstract
Cultivation of human blood monocytes on glass gives rise to cells nonspecifically cytotoxic to tumor cells. If the monocytes are cultured on collagen gels with no contact with glass, no such cytotoxic activity is induced. Killing appeared to be extracellular and probably contact dependent. The glass-induced cytotoxic activity was not related to protein content or cell viability. Rather, it appeared that the monocytes cultured on glass differentiated into cells resembling activated macrophages. On the other hand monocytes cultured on collagen differentiated into cells resembling resident tissue macrophages. These observations are compatible with numerous studies carried out in rodents, showing that activated macrophages, and not resident cells, are cytotoxic to tumor cells.
摘要
在玻璃上培养人血单核细胞会产生对肿瘤细胞具有非特异性细胞毒性的细胞。如果单核细胞在不与玻璃接触的胶原凝胶上培养，则不会诱导出这种细胞毒性活性。杀伤作用似乎是细胞外的，可能依赖于接触。玻璃诱导的细胞毒性活性与蛋白质含量或细胞活力无关。相反，在玻璃上培养的单核细胞似乎分化为类似于活化巨噬细胞的细胞。另一方面，在胶原上培养的单核细胞分化为类似于常驻组织巨噬细胞的细胞。这些观察结果与在啮齿动物中进行的大量研究一致，表明活化的巨噬细胞而非常驻细胞对肿瘤细胞具有细胞毒性。
相似文献
1
In vitro differentiation of human monocytes. Monocytes cultured on glass are cytotoxic to tumor cells but monocytes cultured on collagen are not.人单核细胞的体外分化。在玻璃上培养的单核细胞对肿瘤细胞具有细胞毒性，但在胶原蛋白上培养的单核细胞则不具有。
J Exp Med. 1983 Jun 1;157(6):2061-72. doi: 10.1084/jem.157.6.2061.
2
In vitro differentiation of human monocytes. Differences in monocyte phenotypes induced by cultivation on glass or on collagen.人单核细胞的体外分化。在玻璃或胶原蛋白上培养诱导的单核细胞表型差异。
J Exp Med. 1982 Oct 1;156(4):1101-14. doi: 10.1084/jem.156.4.1101.
3
Cytotoxicity to tumour cells induced in human monocytes cultured in vitro in the presence of different sera.
Acta Pathol Microbiol Scand C. 1979 Apr;87C(2):141-9.
4
Study of the dependence of human monocytes and macrophages antitumoral properties upon TNF-alpha expression or release.人类单核细胞和巨噬细胞抗肿瘤特性对肿瘤坏死因子-α表达或释放的依赖性研究。
Anticancer Res. 1990 Jul-Aug;10(4):949-54.
5
6
7
Cytotoxic effector cell function at different stages of human monocyte-macrophage maturation.人单核细胞-巨噬细胞成熟不同阶段的细胞毒性效应细胞功能。
Cancer Res. 1983 Dec;43(12 Pt 1):5931-6.
8
9
Lymphokine-activated human blood monocytes destroy tumor cells but not normal cells under cocultivation conditions.在共培养条件下，淋巴因子激活的人血单核细胞可破坏肿瘤细胞，但不破坏正常细胞。
J Clin Oncol. 1984 Aug;2(8):937-43. doi: 10.1200/JCO.1984.2.8.937.
10
Involvement of tumor necrosis factor in cytotoxicity mediated by human monocytes.肿瘤坏死因子在人单核细胞介导的细胞毒性中的作用。
Nat Immun Cell Growth Regul. 1988;7(5-6):266-79.
引用本文的文献
1
Reverse Onco-Cardiology: What Is the Evidence for Breast Cancer? A Systematic Review of the Literature.反向肿瘤心脏病学：乳腺癌的证据有哪些？系统文献复习。
Int J Mol Sci. 2023 Nov 19;24(22):16500. doi: 10.3390/ijms242216500.
2
Mechanobiology of cancer cell responsiveness to chemotherapy and immunotherapy: Mechanistic insights and biomaterial platforms.癌症细胞对化疗和免疫疗法反应的机械生物学：机制见解和生物材料平台。
Adv Drug Deliv Rev. 2023 May;196:114771. doi: 10.1016/j.addr.2023.114771. Epub 2023 Mar 6.
3
The complex interactions between the cellular and non-cellular components of the brain tumor microenvironmental landscape and their therapeutic implications.脑肿瘤微环境景观中细胞和非细胞成分之间的复杂相互作用及其治疗意义。
Front Oncol. 2022 Oct 6;12:1005069. doi: 10.3389/fonc.2022.1005069. eCollection 2022.
4
A Novel Method for the Production of an Autologous Anti-Inflammatory and Anti-Catabolic Product (Cytorich) from Human Blood: A Prospective Treatment for the COVID-19-Induced Cytokine Storm.一种从人血中产生自体抗炎和抗分解产物（Cytorich）的新方法：用于治疗 COVID-19 诱导的细胞因子风暴的前瞻性治疗方法。
Med Sci Monit. 2021 Nov 19;27:e934365. doi: 10.12659/MSM.934365.
5
Extracellular matrix and its therapeutic potential for cancer treatment.细胞外基质及其在癌症治疗中的治疗潜力。
Signal Transduct Target Ther. 2021 Apr 23;6(1):153. doi: 10.1038/s41392-021-00544-0.
6
Macrophages and Extracellular Matrix in Breast Cancer: Partners in Crime or Protective Allies?乳腺癌中的巨噬细胞与细胞外基质：是犯罪同伙还是保护盟友？
Front Oncol. 2021 Feb 24;11:620773. doi: 10.3389/fonc.2021.620773. eCollection 2021.
7
LncRNA HOTAIR promotes the growth and metastasis of gastric cancer by sponging miR-1277-5p and upregulating COL5A1.长链非编码 RNA HOTAIR 通过海绵吸附 miR-1277-5p 和上调 COL5A1 促进胃癌的生长和转移。
Gastric Cancer. 2020 Nov;23(6):1018-1032. doi: 10.1007/s10120-020-01091-3. Epub 2020 Jun 24.
8
LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis.LOXL2 抑制为巨噬细胞介导的肝纤维化胶原降解铺平道路。
Front Immunol. 2020 Mar 31;11:480. doi: 10.3389/fimmu.2020.00480. eCollection 2020.
9
Extracellular Matrix in the Tumor Microenvironment and Its Impact on Cancer Therapy.肿瘤微环境中的细胞外基质及其对癌症治疗的影响。
Front Mol Biosci. 2020 Jan 31;6:160. doi: 10.3389/fmolb.2019.00160. eCollection 2019.
10
Macrophages: Key orchestrators of a tumor microenvironment defined by therapeutic resistance.巨噬细胞：治疗抵抗定义的肿瘤微环境中的关键协调者。
Mol Immunol. 2019 Jun;110:3-12. doi: 10.1016/j.molimm.2017.12.003. Epub 2017 Dec 19.
本文引用的文献
1
The growth in vitro of single isolated tissue cells.单个分离组织细胞的体外生长。
J Natl Cancer Inst. 1948 Dec;9(3):229-46.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Augmentation of tumoricidal activity of human monocytes and macrophages by lymphokines.淋巴因子增强人单核细胞和巨噬细胞的杀肿瘤活性。
Int J Cancer. 1980 Jun 15;25(6):691-9. doi: 10.1002/ijc.2910250602.
4
THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY.单核吞噬细胞的分化。形态学、细胞化学与生物化学
J Exp Med. 1965 Jan 1;121(1):153-70. doi: 10.1084/jem.121.1.153.
5
In vitro assay of spontaneous cytotoxicity by human monocytes and macrophages against tumor cells.人单核细胞和巨噬细胞对肿瘤细胞自发细胞毒性的体外测定。
J Immunol Methods. 1980;37(3-4):233-47. doi: 10.1016/0022-1759(80)90310-5.
6
7
Degradation of connective tissue matrices by macrophages. I. Proteolysis of elastin, glycoproteins, and collagen by proteinases isolated from macrophages.巨噬细胞对结缔组织基质的降解。I. 从巨噬细胞中分离出的蛋白酶对弹性蛋白、糖蛋白和胶原蛋白的蛋白水解作用。
J Exp Med. 1980 Nov 1;152(5):1340-57. doi: 10.1084/jem.152.5.1340.
8
Role of activated macrophages in antibody-dependent lysis of tumor cells.活化巨噬细胞在肿瘤细胞抗体依赖性裂解中的作用。
J Exp Med. 1980 Jul 1;152(1):183-97. doi: 10.1084/jem.152.1.183.
9
In vitro cytotoxicity of mouse macrophages activated by coculture with syngeneic sarcoma cells.
Scand J Immunol. 1982 Nov;16(5):421-30. doi: 10.1111/j.1365-3083.1982.tb00742.x.
10
In vitro differentiation of human monocytes. Differences in monocyte phenotypes induced by cultivation on glass or on collagen.人单核细胞的体外分化。在玻璃或胶原蛋白上培养诱导的单核细胞表型差异。
J Exp Med. 1982 Oct 1;156(4):1101-14. doi: 10.1084/jem.156.4.1101.
Zotero 插件