Rubio C A, Stormby N, Kock Y, Thomassen P
Gynecol Oncol. 1983 Jun;15(3):391-5. doi: 10.1016/0090-8258(83)90057-4.
A model system consisting of exfoliated abnormal cells and acellular viscous, mucus-like material was used to investigate the effect of the pressure exerted during smearing on the number of abnormal cells present in the preparations. Smears were made with a cotton swab applicator from left to right with parallel strokes, without overlapping smearing and without rotating the instrument of cell collection. Light and heavy pressures done during smearing procedure were recorded by the aid of an automatic scale. By means of the smearing technique, a gradual decrease in the total number of cells was recorded along the pathway of smearing. By using heavy pressure, however, cell accumulation also occurred at the end of the pathway of the last smearing strokes. Smears made with heavy pressure contained 13% more tumor cells than those made with light pressure. It is concluded that the pressure exerted by the operator during smearing may be an important parameter influencing both the number and the distribution of abnormal cells on smear preparations. These considerations may be of significance in screening programs for tumor cell detection.
一个由脱落的异常细胞和无细胞粘性、粘液样物质组成的模型系统被用于研究涂片过程中施加的压力对制片中异常细胞数量的影响。用棉签涂抹器从左到右以平行笔触进行涂片，不重叠涂抹且不旋转细胞采集工具。借助自动秤记录涂片过程中施加的轻压和重压。通过涂片技术，沿涂片路径记录到细胞总数逐渐减少。然而，使用重压时，在最后一次涂片笔触路径的末端也会出现细胞堆积。重压制作的涂片所含肿瘤细胞比轻压制作的涂片多13%。得出的结论是，操作人员在涂片过程中施加的压力可能是影响涂片制片中异常细胞数量和分布的一个重要参数。这些考虑因素在肿瘤细胞检测的筛查项目中可能具有重要意义。
