Rubio C A, Kock Y
Obstet Gynecol. 1981 Jun;57(6):754-8.
A model system of exfoliated normal human cervicovaginal squamous cells, exfoliated rodent tumor cells, and acellular, viscous, mucus-like material was used to investigate the gradient of cell deposition on smear preparations. Smears were made from left to right with parallel strokes, without overlapping smearing and without rotating the instrument of cell collection. With this technique a gradual decrease in the total number of cells was recorded along the pathway of the smear. The proportion of tumor cells, however, increased halfway along the smearing pathway. The midway increase was significant for wooden spatulas or cotton swab applicators but less marked for plastic spatulas. It would appear that normal squamous cervicovaginal cells, because of their large flat surface, attach readily to the flat surface of the slide and become "consumed" from the material carried by the instrument. On the other hand, the relatively smaller round tumor cells are "pushed" forward by the instrument during the smearing procedure. These considerations may be of significance in screening programs for tumor cell detection.
使用一个由脱落的正常人类宫颈阴道鳞状细胞、脱落的啮齿类肿瘤细胞以及无细胞的、粘性的、粘液样物质组成的模型系统，来研究涂片制备上细胞沉积的梯度。涂片从左到右以平行笔触制作，不重叠涂抹且不旋转细胞采集工具。采用这种技术，沿涂片路径记录到细胞总数逐渐减少。然而，肿瘤细胞的比例在涂片路径的中途增加。对于木制刮铲或棉签涂抹器，中途增加是显著的，但对于塑料刮铲则不太明显。似乎正常的宫颈阴道鳞状细胞由于其大的扁平表面，很容易附着在载玻片的平坦表面上，并从工具携带的物质中“消耗”掉。另一方面，相对较小的圆形肿瘤细胞在涂片过程中被工具“向前推”。这些考虑因素在肿瘤细胞检测的筛查程序中可能具有重要意义。
