Rubio C A, Kock Y, Berglund K
Acta Cytol. 1980 Jan-Feb;24(1):49-53.
A model system of 85% exfoliated normal human cervicovaginal squamous cells, 15% exfoliated rodent t-mor cells and acellular, viscous, mucus-like material was used to investigate the distribution of cells on smear preparations. Smears were made by the same individual with wooden spatulas, using traditional smearing motions. The presence or absence of normal squamous cervicovaginal cells and of tumor cells in ten randomly distributed areas was recorded. Smears made with counterclockwise circular motions contained the largest number of tumor cells, and those with zigzag strokes contained the least. Independent of the motion employed, the largest number of tumor cells was found in ring 9 and the lowest in rings 4 and 10. Some of the factors influencing the accumulation of tumor cells in certain areas of the slide may be: unequal pressure of the spatula during smearing, variations in the angle between the instrument and the surface of the slide and the displacement of tumor cells to certain areas of the preparation by overlapping smearing.
使用一个由85%脱落的正常人类宫颈阴道鳞状细胞、15%脱落的啮齿类肿瘤细胞以及无细胞的、粘性的、粘液样物质组成的模型系统来研究涂片制备上细胞的分布情况。涂片由同一个人用木制刮勺以传统的涂片动作制作而成。记录在十个随机分布区域中正常宫颈阴道鳞状细胞和肿瘤细胞的有无情况。以逆时针圆周运动制作的涂片含有最多数量的肿瘤细胞，而以锯齿状涂抹制作的涂片所含肿瘤细胞最少。无论采用何种动作，在第9环发现的肿瘤细胞数量最多，而在第4环和第10环数量最少。影响肿瘤细胞在载玻片某些区域聚集的一些因素可能是：涂片过程中刮勺压力不均、器械与载玻片表面之间角度的变化以及重叠涂片使肿瘤细胞向制备物的某些区域移位。
