Rubio C A, Berglund K, Kock Y, Zetterberg A
Am J Obstet Gynecol. 1980 Aug 1;137(7):843-6. doi: 10.1016/0002-9378(80)90897-2.
A model system of 15% exfoliated tumor (Ehrlich ascites) cells and 85% exfoliated cervicovaginal human squamous cells and acellular viscous mucuslike material was used to investigate the distribution of tumor cells on smear preparations. The smears were taken by the same individual, with plastic spatulas, using traditional smearing motions. The presence or absence of normal squamous cervicovaginal cells and tumor cells was recorded in 400 randomly distributed areas. Smears done with circular motions contained the largest number of tumor cells and those done with back-and-forth strokes, the least. Circular motions apparently should be preferred if the purpose is to transfer to slides the largest possible number of tumor cells. Independently of the motion employed, the largest total number of tumor cells was found in ring 9 and the lowest, in ring 5. When the various motions were taken into account, the largest number of tumor cells was found in ring 9 for smears done with zigzag strokes; the lowest, in ring 5 with clockwise circular motions. Despite these differences, comparative studies indicate that plastic spatuls may induce a more even distribution of tumor cells onto slides than wooden spatulas or cotton swab applicators. We have been unable to confirm that plastic spatuals induce better cytologic preparations than do wooden spatulas or cotton swab applicators.
采用由15%脱落肿瘤（艾氏腹水）细胞、85%脱落宫颈阴道人鳞状细胞和无细胞粘性粘液样物质组成的模型系统，研究肿瘤细胞在涂片制剂上的分布情况。涂片由同一人使用塑料刮片，采用传统涂片动作采集。在400个随机分布区域记录正常宫颈阴道鳞状细胞和肿瘤细胞的有无。采用圆周运动涂片的肿瘤细胞数量最多，采用来回涂抹的最少。如果目的是将尽可能多的肿瘤细胞转移到载玻片上，显然应首选圆周运动。无论采用何种运动方式，在第9环发现的肿瘤细胞总数最多，在第5环最少。考虑到各种运动方式，采用锯齿状涂抹的涂片在第9环发现的肿瘤细胞数量最多；采用顺时针圆周运动的涂片在第5环发现的肿瘤细胞数量最少。尽管存在这些差异，但比较研究表明，与木质刮片或棉签相比，塑料刮片可能会使肿瘤细胞在载玻片上分布得更均匀。我们无法证实塑料刮片比木质刮片或棉签能制作出更好的细胞学制剂。
