Teschke R, Minzlaff M, Oldiges H, Frenzel H
J Cancer Res Clin Oncol. 1983;106(1):58-64. doi: 10.1007/BF00399898.
To study the effect of chronic alcohol consumption on tumor development due to dimethylnitrosamine (DMN) administration, female Sprague-Dawley rats were pair-fed for 3 weeks a nutritionally adequate liquid diet containing either ethanol (36% of total calories) or isocalorically substituted carbohydrates as control diet. Thereafter, the animals were maintained on laboratory chow and tap water ad libitum for another 2 weeks and received 1.5 mg DMN i.p. per day for the first 5 days. This 5-week cycle was repeated three more times. Chronic treatment with an alcohol-containing diet was shown to significantly improve the mean survival time of DMN-treated rats compared with identically treated animals fed the control diet, but the total number of tumors observed under these experimental conditions and the target organ remained virtually unchanged.
为研究长期饮酒对给予二甲基亚硝胺（DMN）后肿瘤发生的影响，将雌性斯普拉格-道利大鼠成对饲养3周，分别给予含乙醇（占总热量的36%）的营养充足的液体饮食或等热量替代碳水化合物的对照饮食。此后，动物随意进食实验室饲料和自来水，持续2周，并在开始的5天内每天腹腔注射1.5mg DMN。这个5周的周期又重复了3次。结果显示，与给予对照饮食的相同处理动物相比，含酒精饮食的长期处理显著提高了DMN处理大鼠的平均存活时间，但在这些实验条件下观察到的肿瘤总数和靶器官基本保持不变。
