Hills B A
Arch Otolaryngol. 1984 Jan;110(1):3-9. doi: 10.1001/archotol.1984.00800270007003.
Thin-layer chromatography has been used to confirm the presence of phospholipids in the eustachian tubes of dogs and rabbits, analysis showing less phosphatidylcholine but more phosphatidylethanolamine than in pulmonary surfactant. Extracts were surface active not only in reducing surface tension at liquid-air interfaces but in adsorption to hydrophilic solids (glass) to render them hydrophobic. According to a standard test of solid-to-solid adhesiveness, these adsorbed monolayers were found to reduce, by up to 94%, the force of adhesion between surfaces glued by albumin identified in the washings and present in middle-ear effusions. This capability of eustachian surfactant to act as a true release agent is discussed for its possible role in the cause of serous otitis, leading to the concept of an adverse protein-surfactant (adhesion:release) ratio.
薄层色谱法已用于证实犬和兔咽鼓管中磷脂的存在，分析表明，与肺表面活性剂相比，磷脂酰胆碱含量较少，但磷脂酰乙醇胺含量较多。提取物不仅在降低液-气界面的表面张力方面具有表面活性，而且在吸附到亲水性固体（玻璃）上使其具有疏水性方面也具有表面活性。根据一项固体与固体粘附性的标准测试，发现这些吸附的单分子层可将在洗涤液中鉴定出的、存在于中耳积液中的白蛋白所粘附的表面之间的粘附力降低多达94%。本文讨论了咽鼓管表面活性剂作为真正释放剂的这种能力在浆液性中耳炎病因中的可能作用，从而引出了不良蛋白质-表面活性剂（粘附：释放）比率的概念。
