Drew P A, Petrucco O M, Shearman D J
Clin Exp Immunol. 1984 Feb;55(2):437-43.
This study reports the presence of a factor in human milk which is cytotoxic to both autologous and heterologous human peripheral blood mononuclear cells (PBMC). The cytotoxic effect was measured by the release of 51Cr from labelled cells or by the inability of PBMC to exclude trypan blue following exposure to milk. The cytotoxic factor was not dialysable or sensitive to heating at 56 degrees C for 30 min, and could not be adsorbed from milk by PBMC. It lysed lymphocytes harvested from colostrum and from autologous or heterologous milk. The cytotoxic factor in the milk was different from the factor found in colostrum which inhibits the proliferation of mitogen stimulated PBMC, but which is not cytotoxic. Testing of serial samples of milk from the same mother indicated that the factor could be detected in the milk after 3-4 days post partum. We conclude that factors cytotoxic for human PBMC appear in human milk early in lactation.
本研究报告称，人乳中存在一种对自体和异源人外周血单个核细胞（PBMC）均具有细胞毒性的因子。细胞毒性效应通过标记细胞中51Cr的释放或PBMC在接触乳汁后无法排除台盼蓝来测定。该细胞毒性因子不可透析，对56℃加热30分钟不敏感，且PBMC无法从乳汁中吸附该因子。它能裂解从初乳以及自体或异源乳汁中收获的淋巴细胞。乳汁中的细胞毒性因子与初乳中发现的抑制有丝分裂原刺激的PBMC增殖但无细胞毒性的因子不同。对同一母亲的乳汁连续样本进行检测表明，产后3 - 4天可在乳汁中检测到该因子。我们得出结论，对人PBMC具有细胞毒性的因子在哺乳期早期出现在人乳中。
