作为与鸟苷衍生物加合物的诱变剂和致癌物的检测与鉴定。
Detection and identification of mutagens and carcinogens as their adducts with guanosine derivatives.
作者信息
Kasai H, Hayami H, Yamaizumi Z, Nishimura S
出版信息
Nucleic Acids Res. 1984 Feb 24;12(4):2127-36. doi: 10.1093/nar/12.4.2127.
Abstract
For use in screening for environmental mutagens and carcinogens, a highly fluorescent derivative of guanosine, 2'-deoxy-2'-(2",3"-dihydro-2",4"-diphenyl-2"-hydroxy-3"-oxo-1"-pyrrol yl) guanosine (FG), was synthesized. When incubated with FG in aqueous solution, mutagens form adducts that can be analyzed with an HPLC-fluorescence detector-system. By this method, mutagens such as glyoxal, methylglyoxal, 2-(2-furyl)-3-(5-nitrofuryl) acrylamide and 4-nitroquinoline-N-oxide, used as model compounds, were detected rapidly with high sensitivity. Reaction with isopropylideneguanosine (IPG), followed by isolation and characterization of the mutagen-IPG-adduct was found to be a useful method for identifying unknown mutagens in crude samples. This method was successfully applied in identification of the mutagens in heated glucose (200 degrees C, 20 min); glyoxal-IPG and 8-hydroxy-IPG were identified in the reaction mixture.
摘要
为用于环境诱变剂和致癌物的筛选，合成了鸟苷的一种高荧光衍生物，即2'-脱氧-2'-(2",3"-二氢-2",4"-二苯基-2"-羟基-3"-氧代-1"-吡咯基)鸟苷(FG)。当在水溶液中与FG一起孵育时，诱变剂会形成加合物，可通过高效液相色谱-荧光检测系统进行分析。通过这种方法，作为模型化合物的乙二醛、甲基乙二醛、2-(2-呋喃基)-3-(5-硝基呋喃基)丙烯酰胺和4-硝基喹啉-N-氧化物等诱变剂能够被快速且高灵敏度地检测出来。发现与亚异丙基鸟苷(IPG)反应，随后分离并表征诱变剂-IPG-加合物，是鉴定粗样品中未知诱变剂的一种有用方法。该方法成功应用于鉴定加热葡萄糖(200℃，20分钟)中的诱变剂；在反应混合物中鉴定出了乙二醛-IPG和8-羟基-IPG。
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