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人结肠肿瘤的短期器官培养。一项细胞分裂动力学研究。

Human colorectal tumours in short-term organ culture. A stathmokinetic study.

作者信息

Pritchett C J, Senior P V, Sunter J P, Watson A J, Appleton D R, Wilson R G

出版信息

Cell Tissue Kinet. 1982 Sep;15(5):555-64. doi: 10.1111/j.1365-2184.1982.tb01577.x.

DOI:10.1111/j.1365-2184.1982.tb01577.x
PMID:7127403
Abstract

Short-term organ culture, using a technique to preserve epithelial/stromal interaction and metabolism, is a useful technique for carrying out kinetic studies on human colorectal carcinoma and adjacent normal mucosa, providing initial perturbations of proliferative indices are allowed to settle. Tumours require 3.0 micrograms/ml vincristine for complete metaphase arrest compared with mucosa, which needs 0.5 microgram/ml, a 6-fold difference. Using a stathmokinetic technique, the birth rate of tumour cells is 10.21 cells/1000 cells per hr, compared with 7.73 cells/1000 cells per hr for mucosa, a statistically significant difference (P less than 0.01).

摘要

短期器官培养采用一种能维持上皮/基质相互作用及代谢的技术，对于开展人结肠直肠癌及相邻正常黏膜的动力学研究是一项有用的技术，前提是允许增殖指数的初始扰动稳定下来。与需要0.5微克/毫升的黏膜相比，肿瘤需要3.0微克/毫升长春新碱才能实现完全中期阻断，相差6倍。采用静止期动力学技术，肿瘤细胞的生成率为每小时10.21个细胞/1000个细胞，而黏膜为每小时7.73个细胞/1000个细胞，差异具有统计学意义（P<0.01）。

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